GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE53913 Query DataSets for GSE53913
Status Public on Dec 17, 2014
Title RNF17 referees ping-pong in mouse testes [RNAseq]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary In mammals, piRNA populations are dynamic throughout male germ cell development. Embryonic piRNAs consist of both primary and secondary species and are mainly directed toward transposons. In meiotic cells, however, the piRNA population is transposon-poor and restricted to primary piRNAs derived from pachytene piRNA clusters. The mechanism controlling which piRNAs are present at each developmental stage is poorly understood. Here we show that RNF17 shapes adult meiotic piRNA content by suppressing the production of secondary piRNAs. In the absence of RNF17, ping-pong (secondary amplification) occurs inappropriately in meiotic cells – aberrantly targeting protein-coding genes and lncRNAs. Our data indicate that RNF17 comprises one component of a “refereeing” mechanism that prevents deleterious activity of the meiotic piRNA pathway by ensuring the selective loading of PIWI proteins with products of meiotic piRNA clusters.
Overall design Examination of transcriptom in heterozygous and homozygous RNF17 adult testes and RNF17 immunoprecipitates
Contributor(s) Wasik KA, Tam O, Hao Y, Falciatori I, Hammell M, Vagin VV, Hannon GJ
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Jan 08, 2014
Last update date May 15, 2019
Contact name Vasily Vagin
Organization name Cold Spring Harbor
Lab Hannon's
Street address 1 Bungtown Rd
City Cold Spring Harbor
State/province NY
ZIP/Postal code 11724
Country USA
Platforms (2)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
GPL16417 Illumina MiSeq (Mus musculus)
Samples (8)
GSM1303467 het RNF17 6 week old adult testis rep1 RNAseq
GSM1303468 hom RNF17 6 week old adult testis rep1 RNAseq
GSM1303469 het RNF17 6 week old adult testis rep2 RNAseq
This SubSeries is part of SuperSeries:
GSE53919 RNF17 blocks promiscuous activity of PIWI proteins in mouse testes
BioProject PRJNA233959
SRA SRP035245

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE53913_Differentially_expressed_genes_in_hom_RNF17_testes.txt.gz 32.0 Kb (ftp)(http) TXT
GSE53913_Genic_reads_in_heterozygouse_RNF17_lysate_and_RNF17_IP.txt.gz 165.7 Kb (ftp)(http) TXT
GSE53913_Genic_reads_in_hom_and_heterozygouse_RNF17_adult_testes.txt.gz 174.6 Kb (ftp)(http) TXT
GSE53913_Genic_reads_in_homozygouse_RNF17_lysate_and_RNF17_IP.txt.gz 169.4 Kb (ftp)(http) TXT
GSE53913_Multiple_transposon_reads_in_heterozygouse_RNF17_lysate_and_RNF17_IP.txt.gz 11.6 Kb (ftp)(http) TXT
GSE53913_Multiple_transposon_reads_in_hom_and_heterozygouse_RNF17_adult_testes.txt.gz 11.7 Kb (ftp)(http) TXT
GSE53913_Multiple_transposon_reads_in_homozygouse_RNF17_lysate_and_RNF17_IP.txt.gz 10.0 Kb (ftp)(http) TXT
GSE53913_Unique_transposon_reads_in_heterozygouse_RNF17_lysate_and_RNF17_IP.txt.gz 14.4 Kb (ftp)(http) TXT
GSE53913_Unique_transposon_reads_in_hom_and_hetrozygouse_RNF17_adult_testes.txt.gz 15.1 Kb (ftp)(http) TXT
GSE53913_Unique_transposon_reads_in_homozygouse_RNF17_lysate_and_RNF17_IP.txt.gz 14.3 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap