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| Status |
Public on Jan 13, 2014 |
| Title |
MARIS: Method for Analyzing RNA following Intracellular Sorting [array] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We've developed a new Method to Analyze RNA following Intracellular Sorting (MARIS) allowing us to carry out gene expression studies on cells sorted based on intracellular immunoflourescence. The purpose of this study is to determine the degree of bias that MARIS introduces on gene expression. We report Illumina microarray gene expression data from human embryonic stem cells differentiated to a stage in which insulin-expressing cells are present. Gene expression data using RNA isolated from live cells is compared to gene expression data using RNA isolated from MARIS processed cells (fixed, permeabilized, antibody stained and mock sorted) to determine the degree of correlation in gene expression between these two biologically identical samples.
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| Overall design |
Human embryonic stem cells are differentiated to a stage in which insulin-expressing cells are present and split into two biologically identical samples. RNA is immediately isolated from one sample using the RNAeasy protocol (live sample). RNA is isolated from the second sample following MARIS (processed sample) with all cells collected after the sort in order to keep the cell type composition between the live and processed samples the same.
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| Contributor(s) |
Hrvatin S, Deng F, O'Donnell CW, Gifford DK, Melton DA |
| Citation(s) |
24594682 |
| |
| Submission date |
Jan 09, 2014 |
| Last update date |
Jan 28, 2016 |
| Contact name |
Sinisa Hrvatin |
| Organization name |
Harvard Medical School
|
| Department |
Neurobiology
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| Lab |
Greenberg Lab
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| Street address |
220 Longwood ave, GB405
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| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
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| Platforms (1) |
| GPL10904 |
Illumina HumanHT-12 V4.0 expression beadchip (gene symbol) |
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| Samples (6)
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| This SubSeries is part of SuperSeries: |
| GSE54179 |
MARIS: Method for Analyzing RNA following Intracellular Sorting |
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| Relations |
| BioProject |
PRJNA234005 |
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