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| Status |
Public on Jul 23, 2014 |
| Title |
Ustilago maydis gene expression after growth on hydrophobic surface plus/minus hydroxy fatty acids |
| Organism |
Mycosarcoma maydis |
| Experiment type |
Expression profiling by array
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| Summary |
Infection-related development of phytopathogenic fungi is initiated by sensing and responding to plant surface cues. This response results in the formation of specialized infection structures, so-called appressoria. To unravel the program inducing appressoria in the biotrophic smut fungus Ustilago maydis, we exposed cells to a hydrophobic surface and the cutin monomer 16-hydroxy hexadecanoic acid. Genome-wide transcriptional profiling under these appressorium-inducing in vitro conditions revealed dramatic transcriptional changes in almost 20% of the genes. Comparisons with the U. maydis sho1 msb2 double mutant, lacking two putative sensors for plant surface cues, revealed that these plasma membrane receptors regulate a small subset of the surface cue-induced genes. These genes comprised mainly secreted proteins including plant cell wall degrading enzymes that facilitate plant penetration and secreted effectors that are essential virulence factors, with functions after penetration. Our data also demonstrate specific effects on two transcription factors that redirect the transcriptional regulatory network towards appressorium formation and plant penetration. Thus, plant surface cues prime U. maydis for biotrophic development.
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| Overall design |
Solopathogenic Ustilago maydis strain AM1 and its derivate AM1Δsho1Δmsb2 were grown to mid-log phase in YEPSL medium and resuspended in 2% YEPSL plus/minus 16-hydroxy hexadecanoic acid (HDA). The cell suspensions were sprayed on hydrophobic surface (Parafilm) and incubated for 12 h. As control, cells were sprayed on hydrophilic glass surface and incubated for 2 h. After RNA extraction Affymetrix microarrays were performed.
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| Contributor(s) |
Lanver D, Berndt P, Kahmann R |
| Citation(s) |
25033195 |
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| Submission date |
Jan 09, 2014 |
| Last update date |
Jul 24, 2014 |
| Contact name |
Regine Kahmann |
| E-mail(s) |
kahmann@mpi-marburg.mpg.de
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| Organization name |
MPI for Terrestrial Microbiology
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| Department |
Organismic Interactions
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| Lab |
Kahmann
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| Street address |
Karl-von-Frisch Str. 10
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| City |
Marburg |
| ZIP/Postal code |
35043 |
| Country |
Germany |
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| Platforms (1) |
| GPL3681 |
[mpiUstilagoa] MPI UstilagoA |
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| Samples (15)
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| GSM1303885 |
AM1 Parafilm 12h, biological rep1 |
| GSM1303886 |
AM1 Parafilm 12h, biological rep2 |
| GSM1303887 |
AM1 Parafilm 12h, biological rep3 |
| GSM1303888 |
AM1 Parafilm+HDA 12h, biological rep1 |
| GSM1303889 |
AM1 Parafilm+HDA 12h, biological rep2 |
| GSM1303890 |
AM1 Parafilm+HDA 12h, biological rep3 |
| GSM1303891 |
dsho1dmsb2 Parafilm 12h, biological rep1 |
| GSM1303892 |
dsho1dmsb2 Parafilm 12h, biological rep2 |
| GSM1303893 |
dsho1dmsb2 Parafilm 12h, biological rep3 |
| GSM1303894 |
dsho1dmsb2 Parafilm+HDA 12h, biological rep1 |
| GSM1303895 |
dsho1dmsb2 Parafilm+HDA 12h, biological rep2 |
| GSM1303896 |
dsho1dmsb2 Parafilm+HDA 12h, biological rep3 |
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| Relations |
| BioProject |
PRJNA234013 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE53947_RAW.tar |
32.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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