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| Status |
Public on Oct 14, 2014 |
| Title |
TOP1α functions on nucleosome density |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
DNA topoisomerases release supercoils in DNA introduced during replication or transcription. How DNA topoisomerases impact transcription in the context of eukaryotic chromatin is poorly understood. In this study, using a floral stem cell model in Arabidopsis, we uncovered a role of TOP1α in Polycomb Group (PcG) protein-mediated histone lysine 27 trimethylation at, and transcriptional repression of, the stem cell maintenance gene WUSCHEL (WUS). The strong genetic interactions between a top1α mutant and mutations in PcG genes and the overwhelming enrichment of PcG targets among genes affected in expression in the top1a mutant revealed a role of TOP1α in PcG-mediated regulation. Intriguingly, not only the repression of some PcG target genes but also the expression of others requires TOP1α. The mechanism that unifies the opposing effects of TOP1α on PcG target genes appears to lie in its role in decreasing nucleosome density. Genome-wide nucleosome mapping shows that TOP1α is required for the depletion of nucleosomes at regulatory regions of genes, which probably allows the binding of factors that either recruit PcG, as we show for AGAMOUS at the WUS locus, or counteract PcG-mediated regulation. This study uncovers a strong and previously unknown connection between TOP1α and PcG.
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| Overall design |
4 samples in total, two replicates from Ler and top1a-2 plants
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| Contributor(s) |
Liu X, Gao L, Chen X |
| Citation(s) |
25070639 |
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| Submission date |
Jan 09, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Lei Gao |
| E-mail(s) |
leigao@szu.edu.cn
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| Organization name |
shenzhen university
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| Department |
College of Life Sciences
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| Street address |
Nanhai Ave 3688
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| City |
shenzhen |
| State/province |
guangdong |
| ZIP/Postal code |
518060 |
| Country |
China |
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| Platforms (1) |
| GPL13222 |
Illumina HiSeq 2000 (Arabidopsis thaliana) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA234027 |
| SRA |
SRP035267 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE53950_RAW.tar |
24.8 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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