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| Status |
Public on Jul 01, 2014 |
| Title |
The effect of BrdU on gene expression profiles of B6C3F1 mice treated with furan |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Furan is a mouse and rat hepatocarcinogen. We sought to determine the dose-dependent changes in gene expression upon exposure of B6C3F1 mice to furan in order to better understand furan’s mode of action.
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| Overall design |
In this study we examined the transcriptional response in liver tissue of female B6C3F1 mice to BrdU treatment (in drinking water for 5 days before sacrifice). This was a toxicogenomic study in which mice were also exposed to 0, 1 or 8 mg/kg bw furan (by oral gavage) for 3 weeks. Mice were sacrificed four hours after the final furan exposure. Each dose group had 4-5 biological replicates. We used a two-colour reference design and SurePrint G3 Mouse GE 8x60K microarrays (Agilent).
Please note that data for all non-BrdU treated animals was previously reported in GEO [GSE48644]. All samples (with or without BrdU) were part of the same randomized block design for the microarrays.
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| Contributor(s) |
Jackson AF, Williams A, Recio L, Yauk CL |
| Citation(s) |
24910943 |
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| Submission date |
Jan 14, 2014 |
| Last update date |
Feb 02, 2018 |
| Contact name |
Anna Francina Jackson |
| E-mail(s) |
francina.jackson@hc-sc.gc.ca
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| Organization name |
Health Canada
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| Department |
Environmental Health Center
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| Lab |
Dr. Carole Yauk
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| Street address |
50 Colombine Drive, Tunney's Pasture
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| City |
Ottawa |
| State/province |
Ontario |
| ZIP/Postal code |
K1A 0K9 |
| Country |
Canada |
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| Platforms (1) |
| GPL10787 |
Agilent-028005 SurePrint G3 Mouse GE 8x60K Microarray (Probe Name version) |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA234538 |
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