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Status |
Public on Jan 17, 2014 |
Title |
Next Generation Sequencing of MCF-7 and MDA-MB-435 cells |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Human breast cancer cells MDA-MB-435 had higher metastatic potential and other aggressive characteristics than MCF-7 cells. Next-generation RNA sequencing (RNA-Seq) was used to clear this concern through comparison the transcriptomic expression profiles of these two cells.
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Overall design |
Total RNA were extracted from MCF-7 and MDA-MB-435S cells, and the polyA+ mRNA was sequenced using Illumina Genome Analyzer IIx. The reads were mapped to RefSeq RNA reference sequences.
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Contributor(s) |
Gao X, Wang C |
Citation missing |
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Submission date |
Jan 15, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Cuihua Wang |
E-mail(s) |
wang_cuihua@gzlab.ac.cn
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Organization name |
Guangzhou Lab
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Street address |
601 Huangpu Ave, Guangzhou
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City |
Guangzhou |
State/province |
Guangdong |
ZIP/Postal code |
510632 |
Country |
China |
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Platforms (1) |
GPL10999 |
Illumina Genome Analyzer IIx (Homo sapiens) |
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Samples (2) |
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Relations |
BioProject |
PRJNA235273 |
SRA |
SRP035461 |