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| Status |
Public on Jan 24, 2014 |
| Title |
Expression data of Brti1 shRNA knockdown and control shRNA in MCF-10A cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Homologous recombination-mediated DNA repair deficiency (HRD) predisposes to cancer development, but also provides therapeutic opportunities Here, we identified an HRD gene signature that robustly predicted HRD status Unexpectedly, concurrent loss of PTEN in BRCA1-deficient cells might extensively rewire the HR repair network and confer resistance to PARP inhibitor, partially through over-expression of TTK We used the HRD gene signature as a drug discovery tool and found several PARP-inhibitor-synergizing agents through the connectivity map Thus gene expression profiling can be used to define the functional status of the HR repair network providing prognostic and therapeutic information
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| Overall design |
Various shRNAs that target genes involved in homologous recombination (HR) were transfected in MCF-10A non-transformed breast cells lines Stable HR gene knockdown MCF-10A cells were seeded 200000 at 10 cm plate Cells were harvested after 48 hours culturing and used for gene expression profiling
The shRNA that target Brit1 genes was transfected in MCF-10A non-transformed breast cell line by lentiviral particles and selected stable Brit1 knockdown MCF-10A cells. Scrambled control shRNA-transfected MCF-10A cells were applying as control. Both stable Brit1 knockdown and control MCF-10A cells were seeded with 2 x 10^5 cells at 10 cm culture plate. Cells were cultured in MCF-10A medium and harvested after 48 hours culturing. mRNA was extracted from collected cells and performing gene expression profiling. Three or four biological replicates were applied.
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| Contributor(s) |
Peng G, Lin CC, Mo W, Dai H, Park Y, Kim S, Mo Q, Peng Y, Siwko S, Hu R, Lee J, Hennessy B, Hanash S, Mills GB, Lin S |
| Citation(s) |
24553445 |
| |
| Submission date |
Jan 21, 2014 |
| Last update date |
Jan 08, 2019 |
| Contact name |
Chun-Jen Curtis Lin |
| E-mail(s) |
cclin@mdanderson.org
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| Organization name |
University of Texas MD Anderson Cancer Center
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| Department |
Systems Biology
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| Lab |
Dr. Shiaw-Yih Lin
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| Street address |
7345 Fannin Street
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| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77054 |
| Country |
USA |
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| Platforms (1) |
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| Samples (7)
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| This SubSeries is part of SuperSeries: |
| GSE54269 |
A Molecular Portrait of the Homologous Recombination DNA Repair via Genome-wide Transcriptome Profiling |
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| Relations |
| BioProject |
PRJNA236214 |
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