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| Status |
Public on Sep 17, 2014 |
| Title |
The pyruvate-tricarboxylic acid cycle node: a focal point of virulence control in the enteric pathogen Yersinia pseudotuberculosis [crp mutant] |
| Organism |
Yersinia pseudotuberculosis |
| Experiment type |
Expression profiling by array
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| Summary |
Whole transcriptome assessment of the Yersinia pseudotuberculosis strain YPIII. The Y. pseudotuberculosis crp regulon was determined in Yersinia minimal minimum developed for the study. Crp is a key regulator coordinating virulence and metabolism.
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| Overall design |
Y. pseudotuberculosis YPIII or the isogenic crp mutant strain were cultivated at 25°C under aeration on a rotary shaker. First pre-cultures were grown in a 1:1 mixture of HAM’s F-12 Nutrient Mixture (Invitrogen, Carlsbad, US) and liquid DMEM medium (Biochrom, Berlin, DE). Second pre-cultures and main cultures were grown in a Yersinia minimal medium (YMM). The analysis comprised three biological replicates for each strain. In addition, samples, taken at three different time points of the exponential growth phase, were used to validate constant expression during the cultivation. Total RNA was extracted using SV Total RNA Isolation System (Promega). The samples were treated with RNase-free DNase (Roche Applied Science) and the quality of the RNA was confirmed by the lack of PCR amplification of the hns gene and by using an Agilent 2100 Bioanalyzer.
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| Contributor(s) |
Heroven AK, Bücker R |
| Citation(s) |
25164818 |
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| Submission date |
Jan 30, 2014 |
| Last update date |
Sep 17, 2014 |
| Contact name |
Ann Kathrin Heroven |
| E-mail(s) |
Annkathrin.Heroven@helmholtz-hzi.de
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| Phone |
+49-(0)53161815705
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| Organization name |
Helmholtz Centre for Infection Research
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| Department |
Molecular Infection Biology
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| Street address |
Inhoffenstr. 7
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| City |
Braunschweig |
| State/province |
Niedersachsen |
| ZIP/Postal code |
38124 |
| Country |
Germany |
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| Platforms (1) |
| GPL15095 |
Agilent-020412 Yersinia pseudotuberculosis YPIII gene expression array |
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| Samples (3) |
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| This SubSeries is part of SuperSeries: |
| GSE54547 |
The pyruvate-tricarboxylic acid cycle node: a focal point of virulence control in the enteric pathogen Yersinia pseudotuberculosis |
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| Relations |
| BioProject |
PRJNA237011 |
