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| Status |
Public on Feb 03, 2014 |
| Title |
Tg(actb2:KIT-D816V) transgenic zebrafish versus wild-type at 28 hpf |
| Organism |
Danio rerio |
| Experiment type |
Expression profiling by array
|
| Summary |
Transcriptional profiling of 28 hpf zebrafish transgenic expressing human KIT-D816V mutant gene versus wild-type embryos was performed. The aim of this experiment was to determine the expression changes caused by expression of KIT-D816V oncogene.
This transgenic zebrafish represents a model of systemic mastocytosis with many features of this disease present in adults, but no very clear phenotypes in embryos. Thus, we attempted to determine if there are certain expression changes, which can be used as molecular features of active KIT expressed in these embryos.
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| Overall design |
Two-condition experiment, Tg(actb2:KIT-D816V) versus wild-type(AB), 4 replicate experiments with a dye-swap each time
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| Contributor(s) |
Balci TB, Prykhozhij SV, Berman JN |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 02, 2014 |
| Last update date |
Dec 17, 2015 |
| Contact name |
Sergey V Prykhozhij |
| E-mail(s) |
s.prykhozhij@gmail.com
|
| Phone |
9028178846
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| Organization name |
CHEO Research Institute/University of Ottawa
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| Lab |
Berman Lab
|
| Street address |
Room 318/319 | CAREG, 20 Marie-Curie Private
|
| City |
Ottawa |
| State/province |
Ontario |
| ZIP/Postal code |
K1N 9B4 |
| Country |
Canada |
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| Platforms (1) |
| GPL14664 |
Agilent-026437 D. rerio (Zebrafish) Oligo Microarray V3 (Probe Name version) |
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| Samples (8)
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| GSM1319940 |
RNA from KIT-D816V transgenics (labeled Cy3) hybridized together with wild-type RNA (labeled Cy5) - replicate 1 |
| GSM1319941 |
RNA from KIT-D816V transgenics (labeled Cy3) hybridized together with wild-type RNA (labeled Cy5) - replicate 2 |
| GSM1319942 |
RNA from KIT-D816V transgenics (labeled Cy3) hybridized together with wild-type RNA (labeled Cy5) - replicate 3 |
| GSM1319943 |
RNA from KIT-D816V transgenics (labeled Cy3) hybridized together with wild-type RNA (labeled Cy5) - replicate 4 |
| GSM1319944 |
RNA from KIT-D816V transgenics (labeled Cy5) hybridized together with wild-type RNA (labeled Cy3) - replicate 1 |
| GSM1319945 |
RNA from KIT-D816V transgenics (labeled Cy5) hybridized together with wild-type RNA (labeled Cy3) - replicate 2 |
| GSM1319946 |
RNA from KIT-D816V transgenics (labeled Cy5) hybridized together with wild-type RNA (labeled Cy3) - replicate 3 |
| GSM1319947 |
RNA from KIT-D816V transgenics (labeled Cy5) hybridized together with wild-type RNA (labeled Cy3) - replicate 4 |
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| This SubSeries is part of SuperSeries: |
| GSE54615 |
Microarray analyses of transgenic zebrafish expressing human KIT-D816V gene versus wild-type zebrafish |
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| Relations |
| BioProject |
PRJNA237187 |
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