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| Status |
Public on Feb 06, 2014 |
| Title |
RNase J is required for processing of a small number of RNAs in Rhodobacter sphaeroides |
| Organism |
Cereibacter sphaeroides 2.4.1 |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
A comparative RNA-seq approach between R. sphaeroides 2.4.1 wild type and an RNase J deletion strain 2.4.1∆rnj revealed the accumulation of different mRNA fragments in the mutant. The structural characteristics of these RNA fragments suggest that RNase J is responsible for the decay of degradation intermediates that cannot serve as substrates for the 3´-to-5´ exoribonucleases.
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| Overall design |
Total RNA from exponentially grown cultures (OD660 0,4) of 2.4.1 wild type and 2.4.1∆rnj has been isolated in triplicates using the hot phenol method and prepared for sequencing on a Illumina Genome Analyzer IIx machine.
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| Contributor(s) |
Rische-Grahl T, Weber L, Remes B, Förstner KU, Klug G |
| Citation(s) |
24922065 |
| |
| Submission date |
Feb 06, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Konrad U. Förstner |
| E-mail(s) |
foerstner@zbmed.de
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| Organization name |
ZB MED - Information Centre for Life Sciences
|
| Department |
Information Services
|
| Lab |
Förstner Lab
|
| Street address |
Gleueler Str. 60
|
| City |
Cologne |
| State/province |
North Rhine-Westphalia |
| ZIP/Postal code |
50931 |
| Country |
Germany |
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| Platforms (1) |
| GPL18261 |
Illumina Genome Analyzer IIx (Rhodobacter sphaeroides 2.4.1) |
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| Samples (2) |
| GSM1323506 |
Rhodobacter sphaeroides wild type |
| GSM1323507 |
Rhodobacter sphaeroides RNase J deletion mutant |
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| Relations |
| BioProject |
PRJNA237547 |
| SRA |
SRP036835 |
