|
| Status |
Public on Feb 19, 2015 |
| Title |
SCML2 Establishes the Male Germline Epigenome |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Gametogenesis is dependent on the expression of germline-specific genes. However, it remains unknown how the germline epigenome is distinctly established from that of somatic lineages. Here we show that genes commonly expressed in somatic lineages and spermatogenesis-progenitor cells undergo repression in a genome-wide manner in late stages of the male germline and identify underlying mechanisms. SCML2, a germline-specific subunit of a Polycomb repressive complex 1 (PRC1), establishes the unique epigenome of the male germline through two distinct antithetical mechanisms. SCML2 works with PRC1 and promotes RNF2-dependent ubiquitination of H2A, thereby marking somatic/progenitor genes on autosomes for repression. Paradoxically, SCML2 also prevents RNF2-dependent ubiquitination of H2A on sex chromosomes during meiosis, thereby enabling unique epigenetic programming of sex chromosomes for male reproduction. Our results reveal divergent mechanisms involving a shared regulator by which the male germline epigenome is distinguished from that of the soma and progenitor cells.
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| |
|
| Overall design |
RNA-seq and ChIP-seq analyses using wild-type and Scml2-KO spermatogenic cells
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| |
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| Contributor(s) |
Hasegawa K, Sin HS, Maezawa S, Kartashov AV, Alavattam KG, Bacon WC, Barski A, Namekawa SH |
| Citation(s) |
25703348, 26198001 |
| |
| Submission date |
Feb 14, 2014 |
| Last update date |
Oct 10, 2019 |
| Contact name |
Satoshi Namekawa |
| E-mail(s) |
satoshi.namekawa@cchmc.org
|
| Phone |
513-803-1377
|
| Organization name |
Cincinnati Children's Hospital Medical Center
|
| Street address |
3333 Burnet Ave
|
| City |
Cincinnati |
| State/province |
Ohio |
| ZIP/Postal code |
45229 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
|
| Samples (28)
|
|
| Relations |
| BioProject |
PRJNA238350 |
| SRA |
SRP037992 |
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