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Series GSE554 Query DataSets for GSE554
Status Public on Dec 25, 2003
Title Mesangioblast clone comparison- U74B
Organism Mus musculus
Experiment type Expression profiling by array
Summary Mouse dorsal aorta from E9.5 mouse embryos (C57BL6) was grown as an explant culture as described elsewhere (De Angelis et al., 1999). After 5 days the explant was dissociated into a single cell suspension, and plated at limiting dilution on a feeder layer of Mitomycin C-treated primary embryonic mouse fibroblasts, in 96 multiwell plates in complete medium, RPMI 20% Fetal Calf Serum. After 1 week, mesangioblast clones appeared approximately 2-4% of the wells. When the clones had grown to approximately 103 cells they were passed on gelatin-coated dishes, and further expanded. Clones D16 and D351 were used for microarray analysis.
Keywords: other
 
 
Contributor(s) Brunelli S, Tagliafico E, De Angelis F, Tonlorenzi R, Baesso S, Ferrari S, Bozzoni I, Ferrari S, Cossu G
Citation(s) 15155529
Submission date Jul 23, 2003
Last update date Jul 06, 2016
Contact name Enrico Tagliafico
E-mail(s) enrico.tagliafico@unimore.it
Organization name University of Modena and Reggio Emilia
Department Center for Genome Research
Street address Via Campi 287
City Modena
State/province MO
ZIP/Postal code 41126
Country Italy
 
Platforms (1)
GPL82 [MG_U74Bv2] Affymetrix Murine Genome U74B Version 2 Array
Samples (2)
GSM8511 D16 mesoangioblast clone U74B
GSM8514 D351 mesoangioblast clone U74B
Relations
BioProject PRJNA85315

D351vsD16 mesoangioblast clone U74B header descriptions
ID_REF
VALUE Affymetrix MAS 5.0 Signal Log Ratio value (vs baseline)
Change Affymetrix MAS 5.0 Change value (vs baseline)
Change p-value Affymetrix MAS 5.0 Change p-value (vs baseline)

Data table
ID_REF VALUE Change Change p-value
AFFX-MurIL2_at 1.8 NC 0.5
AFFX-MurIL10_at -2.1 NC 0.8465
AFFX-MurIL4_at -1.9 NC 0.5
AFFX-MurFAS_at 1.9 I 0
AFFX-BioB-5_at -0.5 NC 0.984246
AFFX-BioB-M_at -0.7 D 0.999996
AFFX-BioB-3_at -0.6 D 0.998306
AFFX-BioC-5_at -0.7 D 0.999983
AFFX-BioC-3_at -0.7 D 0.999977
AFFX-BioDn-5_at -0.6 D 0.999925
AFFX-BioDn-3_at -0.7 D 1
AFFX-CreX-5_at -0.6 D 0.999998
AFFX-CreX-3_at -0.5 D 1
AFFX-BioB-5_st 0.4 NC 0.5
AFFX-BioB-M_st 0.1 NC 0.91689
AFFX-BioB-3_st -0.8 NC 0.569357
AFFX-BioC-5_st -0.1 NC 0.5
AFFX-BioC-3_st -0.6 NC 0.949481
AFFX-BioDn-5_st -0.2 NC 0.5
AFFX-BioDn-3_st -0.3 NC 0.5

Total number of rows: 12477

Table truncated, full table size 303 Kbytes.




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