S. Typhimurium 112910a parental strain and an isogenic ΔscsA strain were grown to stationary phase in SPI2 inducing minimal medium (MM5.8 - Erikkson et al., 2006 Inf & Imm., 74:1243) with or without supplementation with 1µM cortisol
Overall design
4 samples (stat phase wt, stat phase wt + 1µM cortisol, stat phase ΔscsA, stat phase ΔscsA + 1µM cortisol) and 3 biological replicates for each sample.