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| Status |
Public on Feb 14, 2017 |
| Title |
Bmi1 defines long-term adult cardiac stem cells in heart homeostasis and repair |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We have found the existence of a Bmi1+ population in the adult heart contributing to the organ low-rate turnover and repair with the generation of new cardiomyocytes. We show that the Bmi1+ population is a sub-population of the cardiac Sca-1+ progenitor cells. We have analyzed the gene profile by deep-sequencing (RNA-Seq) of Bmi1+ and Sca-1+Bmi1- cells in homeostatic heart condition. On the other hand, we have compared gene profile by deep-sequencing (RNA-Seq) of Bmi1+ cells in homeostatic condition versus Bmi1+ cells 5 days after myocardial infarction (MI). Analysis of RNA-Seq data revealed a differential expression signature between both subsets of cardiac stem/progenitors cells in homeostatic condition and also differences between Bmi1+ cells after AMI versus homeostatic condition.
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| Overall design |
Examination of gene profile of 2 different cardiac stem /progenitors subsets (Bmi1+ and Sca-1+Bmi1-) co-existing inthe adult heart under steady state. Examination of gene profile of Bmi1+ cardiac stem cells in homeostatic condition versus MI
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| Contributor(s) |
Bernad A |
| Citation(s) |
27472922, 30851670 |
| |
| Submission date |
Mar 10, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Antonio Bernad |
| E-mail(s) |
abernad@cnic.es
|
| Organization name |
Spanish Cardiovascular Research Center
|
| Street address |
Melchor Fernandez Almagro, 3
|
| City |
Madrid |
| ZIP/Postal code |
28029 |
| Country |
Spain |
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| Platforms (1) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA240889 |
| SRA |
SRP039628 |
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