|
| Status |
Public on Mar 14, 2014 |
| Title |
Low-density lipoprotein receptor-5 contributes to whole body metabolism by regulating fatty acid metabolism in the osteoblast |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Wnt signaling is critical for normal skeletal development as well as whole-body metabolic function. In this study, we described a previously unrecognized function for Wnt-Lrp5 signaling in the osteoblast that allows bone to acquire the resources necessary to fuel bone formation. Mice lacking the Lrp5 co-receptor specifically in osteoblasts and osteocytes exhibit the expected reductions in postnatal bone mass and also develop peripheral adiposity with corresponding reductions in energy expenditure. Conversely, mice expressing a high-bone mass mutant Lrp5 allele are leaner with reduced plasma triglyceride and free fatty acid levels. In this context, Wnt-initiated signals downstream of Lrp5, but not Lrp6, regulate the expression of key enzymes required for fatty acid β-oxidation. These results suggest that Wnt-Lrp5 signaling regulates basic cellular activities beyond those associated with fate-specification and differentiation in bone and that the skeleton influences global energy homeostasis via mechanisms independent of osteocalcin and glucose metabolism
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| |
|
| Overall design |
Total RNA isolated from cultures of Lrp5-deficient osteoblasts differentiated for 7 days in vitro compared to control osteoblasts
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| |
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| Contributor(s) |
Riddle RC, Farber CR |
| Citation(s) |
25802278 |
| Submission date |
Mar 13, 2014 |
| Last update date |
Jun 14, 2018 |
| Contact name |
Charles R Farber |
| E-mail(s) |
crf2s@virginia.edu
|
| Organization name |
University of Virginia
|
| Department |
Center for Public Health Genomics
|
| Street address |
P.O. Box 800717
|
| City |
Charlottesville |
| State/province |
VA |
| ZIP/Postal code |
22908 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
|
| Samples (8)
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| Relations |
| BioProject |
PRJNA241275 |
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