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Series GSE55930 Query DataSets for GSE55930
Status Public on Dec 31, 2014
Title Drug treatment of colorectal and endometrial cancer cell lines
Organism Homo sapiens
Experiment type Non-coding RNA profiling by array
Summary We used expression profiling of colorectal cancer and endometrial cancer cell lines treated with demethylating agents to search for epigenetically regulated miRNAs.
The study included three MMR-deficient colorectal cancer cell lines (HCT116, HCT15, and RKO), two MMR-proficient colorectal cancer cell lines (SW480, and T84) and two MMR-deficient endometrial cancer cell lines (AN3CA and HEC59).
 
Overall design Cancer cell lines were cultured and divided to two parts. One part of cell culture was treated with demethylating agents (5 uM 5-aza-2' deoxycytidine, Sigma A3656, for 96 hours and with 300 nM trichostatin, Sigma T1952, for 18 hours).
The other part of cancer cell line was cultured without treatments. All treatments were performed in duplicates.
 
Contributor(s) Kaur S, Lotsari JE
Citation(s) 25767621
Submission date Mar 14, 2014
Last update date Oct 17, 2019
Contact name Johanna Lotsari-Salomaa
E-mail(s) johanna.lotsari@helsinki.fi
Phone +358408286827
Organization name University of Helsinki
Department Department of Medical Genetics
Street address Haartmaninkatu 8
City Helsinki
ZIP/Postal code 00014
Country Finland
 
Platforms (1)
GPL16541 Agilent-019118 Human miRNA Microarray 2.0 (miRNA_107_Sep09)
Samples (34)
GSM1348670 HCT15 human colon adenocarcinoma, treated cell line 1
GSM1348671 HCT15 human colon adenocarcinoma, treated cell line 2
GSM1348672 HCT15 human colon adenocarcinoma, untreated cell line 1
Relations
BioProject PRJNA241338

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE55930_140314_normalized_miRNA_data.txt.gz 77.7 Kb (ftp)(http) TXT
GSE55930_RAW.tar 25.6 Mb (http)(custom) TAR (of TXT)
GSE55930_Supplementary_Table_3_Upregulated.txt.gz 2.3 Kb (ftp)(http) TXT
Processed data are available on Series record
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