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Series GSE5594 Query DataSets for GSE5594
Status Public on Sep 19, 2007
Title Acetaminophen (APAP) Rat Blood Test Gene Expression Data Set
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Gene expression test data set from rat blood samples exposed to either 150, 1500 or 2000 mg/kg of APAP for 3, 6 or 24 hours.

The Supplementary file (appended below) contains the mapping for the decoding of blinded samples.
Keywords: Dose response, Time course, Microarray, Gene expression
 
Overall design Male F344/N rats, 10-12 weeks old, were exposed to 0 (vehicle only), 150, 1500 or 2000 mg/kg APAP in 0.5% ethyl cellulose by oral gavage in two doses to increase absorption. The animals were sacrificed after 3, 6, or 24 hours. Equal amounts of RNA from the blood from each of six vehicle-only treated control animals were pooled at each time point and compared to individual control and treated animals at each dose and time period. The samples were hybridized in duplicate for each individual rat for a total of 12 (test set) microarray chips per dose and time period.

Experiments were performed according to the guidelines established in the NIH Guide for the Care and Use of Laboratory Animals and an approved Animal Study Protocol was on file prior to initiation of the study.
 
Contributor(s) Bushel PR, Heinloth AN, Paules RS
Citation(s) 17984051
Submission date Aug 24, 2006
Last update date Dec 06, 2012
Contact name Pierre Robert Bushel
E-mail(s) bushel@niehs.nih.gov
Phone 919-316-4564
Organization name NIEHS
Department Biostatistics and Computational Biology Branch
Lab Bioinformatics
Street address P.O. Box 12233
City RTP
State/province NC
ZIP/Postal code 27709
Country USA
 
Platforms (1)
GPL890 Agilent-011868 Rat Oligo Microarray G4130A (Feature Number version)
Samples (144)
GSM129907 Acetaminophen 24hr Rat Pooled Control blood vs. Acetaminophen 24hr Rat 5480 blood
GSM129908 Acetaminophen 3hr Rat Pooled Control blood vs. Acetaminophen 3hr Rat 8846 blood
GSM129909 Acetaminophen 6hr Rat Pooled Control blood vs. Acetaminophen 6hr Rat 3763 blood
This SubSeries is part of SuperSeries:
GSE5652 Blood and liver exposed to acetaminophen (APAP)
Relations
BioProject PRJNA104359

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE5594_RAW.tar 2.8 Mb (http)(custom) TAR
GSE5594_blood_test_decoder.txt 2.4 Kb (ftp)(http) TXT

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