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Series GSE55945 Query DataSets for GSE55945
Status Public on Mar 18, 2014
Title Gene Expression Profiling of Prostate Benign and Malignant Tissue
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We profiled genome-wide gene expression of human prostate benign and malignant tissue to identify potential biomarkers and immunotherapy targets.
We stratified malignant specimens according to their TMPRSS2:ERG gene fusion status.
 
Overall design Radical prostatectomy tissue samples were obtained from the Hershey Foundation Prostate Cancer Serum and Tumor Bank at our institution. Morphologic diagnosis was done by a pathologist. OCT blocks containing >30% of PCa tissue (with Gleason score of 6 or 7) were selected for RNA purification. A biopsy punch was used to select the PCa tissues from the OCT sample blocks. Benign or PCa tissues were homogenized using a TissueLyser (Qiagen) at 28 Hz for 5 min. Total RNA was isolated using Trizol reagent. RNA was quantified by NanoDrop ND-1000 spectrophotometer, and quality was evaluated with Agilent RNA 6000 NanoChip and the 2100 Bioanalyzer, with 28S/18S ratios and RIN determined by 2100 Expert software.
 
Contributor(s) Arredouani MS, Lu B, Sanda M
Citation(s) 19737960
Submission date Mar 17, 2014
Last update date Mar 25, 2019
Contact name M. Simo Arredouani
E-mail(s) simarred@gmail.com
Organization name Beth Israel Deaconess Medical Center
Department Surgery
Street address 330 Brookline Ave.
City Boston
State/province MA
ZIP/Postal code 02215
Country USA
 
Platforms (1)
GPL570 [HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array
Samples (21)
GSM1348933 Prostate Cancer_MS 36D6
GSM1348934 Prostate Cancer_MS 36C1
GSM1348935 Prostate Cancer_MS 36D7
Relations
BioProject PRJNA241405

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE55945_RAW.tar 96.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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