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Series GSE5595 Query DataSets for GSE5595
Status Public on Sep 19, 2007
Title Acetaminophen (APAP) Rat Liver Test Gene Expression Data Set
Organism Rattus norvegicus
Experiment type Expression profiling by array
Summary Gene expression test data set from rat liver samples exposed to either 150, 1500 or 2000 mg/kg of APAP for 3, 6 or 24 hours.

The Supplementary file (appended below) contains the mapping for the decoding of blinded samples.
Keywords: Dose response, Time course, Microarray, Gene expression
 
Overall design Male F344/N rats, 10-12 weeks old, were exposed to 0 (vehicle only), 150, 1500 or 2000 mg/kg APAP in 0.5% ethyl cellulose by oral gavage in two doses to increase absorption. The animals were sacrificed after 3, 6, or 24 hours. At the time of sacrifice, liver sections from the left lobe were isolated, cubed, flash frozen and pulverized. Total hepatic RNA was isolated from individual rat livers. Equal amounts of RNA from the liver from each of six vehicle-only treated control animals were pooled at each time point and compared to individual control and treated animals at each dose and time period. The samples were hybridized in duplicate for each individual rat for a total of 12 (test set) microarray chips per dose and time period.

Experiments were performed according to the guidelines established in the NIH Guide for the Care and Use of Laboratory Animals and an approved Animal Study Protocol was on file prior to initiation of the study.
 
Contributor(s) Bushel PR, Heinloth AN, Paules RS
Citation(s) 17984051
Submission date Aug 24, 2006
Last update date Dec 06, 2012
Contact name Pierre Robert Bushel
E-mail(s) bushel@niehs.nih.gov
Phone 919-316-4564
Organization name NIEHS
Department Biostatistics and Computational Biology Branch
Lab Bioinformatics
Street address P.O. Box 12233
City RTP
State/province NC
ZIP/Postal code 27709
Country USA
 
Platforms (1)
GPL890 Agilent-011868 Rat Oligo Microarray G4130A (Feature Number version)
Samples (144)
GSM130058 Control Acetaminophen 3hr Rat left_liver_lobe Pool vs. Acetaminophen 3hr Rat 4623 left_liver_lobe
GSM130059 Control Acetaminophen 24hr Rat left_liver_lobe Pool vs. Acetaminophen 24hr Rat 4678 left_liver_lobe
GSM130060 Control Acetaminophen 3hr Rat left_liver_lobe Pool vs. Acetaminophen 3hr Rat 5339 left_liver_lobe
This SubSeries is part of SuperSeries:
GSE5652 Blood and liver exposed to acetaminophen (APAP)
Relations
BioProject PRJNA104361

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE5595_Liver_test_decoder.txt 2.4 Kb (ftp)(http) TXT
GSE5595_RAW.tar 2.8 Mb (http)(custom) TAR

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