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| Status |
Public on Mar 19, 2014 |
| Title |
mRNA profile in DR-related mutants |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Dietary restriction (DR) extends lifespan in a wide variety of species, yet the underlying mechanisms are not well understood. Here we show that the Caenorhabditis elegans HNF4α-related nuclear hormone receptor NHR-62 is required for metabolic and physiologic responses associated with DR-induced longevity. nhr-62 mediates the longevity of eat-2 mutants, a genetic mimetic of dietary restriction, and blunts the longevity response of DR induced by bacterial food dilution at low nutrient levels. Metabolic changes associated with DR, including decreased Oil Red O staining, decreased triglyceride levels, and increased autophagy are partly reversed by mutation of nhr-62. Additionally, the DR fatty acid profile is altered in nhr-62mutants. Expression profiles reveal that several hundred genes induced by DR depend on the activity of NHR-62, including a putative lipase required for the DR response. This study provides critical evidence of nuclear hormone receptor regulation of the DR longevity response, suggesting hormonal and metabolic control of life span.
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| Overall design |
Young adult worms before bearing eggs inside were collected. N2 serves as the control of wild type. 3 biological replicates included in this experiment.
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| Contributor(s) |
Shen Y, Antebi A |
| Citation(s) |
23935515 |
| |
| Submission date |
Mar 18, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Corinna Klein |
| E-mail(s) |
cklein@age.mpg.de
|
| Organization name |
Max Planck Institute for Biology of Ageing
|
| Street address |
Joseph-Stelzmann-Straße 9b
|
| City |
Köln |
| ZIP/Postal code |
50931 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL18245 |
Illumina HiSeq 2500 (Caenorhabditis elegans) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA241944 |
| SRA |
SRP040269 |
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