|
| Status |
Public on Oct 23, 2014 |
| Title |
White Cells Facilitate Opposite- and Same-Sex Mating of Opaque Cells in Candida albicans |
| Organism |
Candida albicans |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Modes of sexual reproduction in eukaryotic organisms are highly diversified. The human fungal pathogen Candida albicans undergoes a phenotypic switch from the white to the opaque phase in order to become mating-competent. In this study, we report that functionally and morphologically differentiated white and opaque cells show a coordinating behavior in the process of mating. Although white cells are mating-incompetent, they are induced to produce sexual pheromones when treated with opposite pheromones or interacted with opaque cells of an opposite mating type. In a co-culture system, pheromones released by white cells induce opaque cells to form mating projections and thus facilitate both opposite- and same-sex mating of opaque cells. Deletion of genes encoding the pheromone precursor proteins and inactivation of the pheromone response signaling pathway (Ste2-MAPK-Cph1) impair the promoting role of white cells (MTLa) in sexual mating of opaque cells. White and opaque cells communicate via a paracrine pheromone signaling and thus create an environment conducive to sexual mating. This coordination behavior of the two different cell types may be a trade-off strategy between sexual and asexual lifestyles in C. albicans.
|
| |
|
| Overall design |
total RNA profiles of white cell treated with pheromone
|
| |
|
| Contributor(s) |
Huang G, Tao L, Cao C |
| Citation(s) |
25329547 |
| |
| Submission date |
Mar 19, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
guanghua huang |
| Organization name |
Institute of Microbiology
|
| Street address |
NO.1 West Beichen Road, Chaoyang District
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL15645 |
Illumina HiSeq 2000 (Candida albicans) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA242279 |
| SRA |
SRP040313 |
