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| Status |
Public on Jun 01, 2015 |
| Title |
Microarray analysis of a Drosophila dopamine transporter mutant, fumin (fmn) |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by array
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| Summary |
We previously found a short sleeper mutant, fmn, and identified its mutation in the dopamine transporter gene. In an attempt to discover additional sleep related genes in Drosophila, we carried out a microarray analysis comparing mRNA expression in heads of fmn and control flies and found differentially expressed genes.
Next, using the pan-neuronal Gal4 driver elav-Gal4 and UAS-RNA interference (RNAi) to knockdown individual genes, we performed a functional screen. We found that knockdown of the NMDA type glutamate receptor channel gene (Nmdar1) reduced sleep.
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| Overall design |
Control (w1118) and fmn flies collected every 4 h at seven time points under 12 h of light (zeitgeber time, ZT 0-12) followed by 12 h darkness (ZT 12-24) (LD) were analyzed.
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| Contributor(s) |
Tomita J, Kume K |
| Citation(s) |
26023770 |
| Submission date |
Mar 24, 2014 |
| Last update date |
May 04, 2018 |
| Contact name |
Jun Tomita |
| Organization name |
Nagoya City University
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| Street address |
3-1 Tanabe, Mizuho
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| City |
Nagoya |
| ZIP/Postal code |
467-8603 |
| Country |
Japan |
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| Platforms (1) |
| GPL1322 |
[Drosophila_2] Affymetrix Drosophila Genome 2.0 Array |
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| Samples (14)
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| Relations |
| BioProject |
PRJNA242556 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE56149_RAW.tar |
30.1 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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