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| Status |
Public on May 09, 2014 |
| Title |
Macrophage-restricted Interleukin-10 receptor-, but not IL-10 deficiency causes severe spontaneous colitis |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Interleukin-10 (IL-10) is a pleiotropic anti-inflammatory cytokine produced and sensed by most hematopoietic cells. Genome wide association studies and experimental animal models point at a central role of the IL-10 axis in Inflammatory Bowel Diseases. Here we investigated the importance of intestinal macrophage production of IL-10 and their IL-10 exposure, as well as the existence of an IL-10-based autocrine regulatory loop in the gut. Specifically, we generated mice harboring IL-10 or IL-10 receptor (IL-10Rα) mutations in intestinal lamina propria-resident chemokine receptor CX3CR1hi-expressingmacrophages. We found macrophage-derived IL-10 dispensable for gut homeostasis and maintenance of colonic T regulatory cells. In contrast, loss of IL-10 receptor expression impaired the critical conditioning of these monocyte-derived macrophages, but resulted in spontaneous development of severe colitis. Collectively, our results highlight IL-10 as a critical homeostatic macrophage-conditioning factor in the colon and define intestinal CX3CR1hi macrophages as a decisive factor that determines gut health or inflammation.
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| Overall design |
Microarray of resident macrophages sorted from colons of Interleukin-10 deficeint mice and macrophage-restricted interleukin-10 receptor deficient mice versus colonic resident macrophages of wild type mice
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| Contributor(s) |
Zigmond E, Bernshtein B, Friedlander G, Walker CR, Yona S, Kim K, Brenner O, Krauthgamer R, Varol C, Mueller W, Jung S |
| Citation(s) |
24792913 |
| |
| Submission date |
Apr 02, 2014 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Gilgi Friedlander |
| Organization name |
Weizmann Institute of Science
|
| Street address |
Hertzel st
|
| City |
Rehovot |
| ZIP/Postal code |
76100 |
| Country |
Israel |
| |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (7)
|
| GSM1361638 |
FACS sorted CX3CR1hi macrophages from the colons of 6 weeks old CX3CR1gfp/+ mice_SJ38 |
| GSM1361639 |
FACS sorted CX3CR1hi macrophages from the colons of 6 weeks old IL10-/- CX3CR1gfp/+ mice_SJ41 |
| GSM1361640 |
FACS sorted resident macrophages from the colons of 6 weeks old CX3CR1gfp/+ mice_SJ55 |
| GSM1361641 |
FACS sorted resident macrophages from the colons of 6 weeks old CX3CR1gfp/+ mice_SJ56 |
| GSM1361642 |
FACS sorted resident macrophages from the colons of 6 weeks old CX3CR1gfp/+ mice_SJ68 |
| GSM1361643 |
FACS sorted resident macrophages from the colons of 6 weeks old CX3CR1cre:IL10Raflox/flox mice_SJ54 |
| GSM1361644 |
FACS sorted resident macrophages from the colons of 6 weeks old CX3CR1cre:IL10Raflox/flox mice_SJ67 |
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| Relations |
| BioProject |
PRJNA243329 |
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