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Series GSE56444 Query DataSets for GSE56444
Status Public on May 09, 2014
Title Macrophage-restricted Interleukin-10 receptor-, but not IL-10 deficiency causes severe spontaneous colitis
Organism Mus musculus
Experiment type Expression profiling by array
Summary Interleukin-10 (IL-10) is a pleiotropic anti-inflammatory cytokine produced and sensed by most hematopoietic cells. Genome wide association studies and experimental animal models point at a central role of the IL-10 axis in Inflammatory Bowel Diseases. Here we investigated the importance of intestinal macrophage production of IL-10 and their IL-10 exposure, as well as the existence of an IL-10-based autocrine regulatory loop in the gut. Specifically, we generated mice harboring IL-10 or IL-10 receptor (IL-10Rα) mutations in intestinal lamina propria-resident chemokine receptor CX3CR1hi-expressingmacrophages. We found macrophage-derived IL-10 dispensable for gut homeostasis and maintenance of colonic T regulatory cells. In contrast, loss of IL-10 receptor expression impaired the critical conditioning of these monocyte-derived macrophages, but resulted in spontaneous development of severe colitis. Collectively, our results highlight IL-10 as a critical homeostatic macrophage-conditioning factor in the colon and define intestinal CX3CR1hi macrophages as a decisive factor that determines gut health or inflammation.
Overall design Microarray of resident macrophages sorted from colons of Interleukin-10 deficeint mice and macrophage-restricted interleukin-10 receptor deficient mice versus colonic resident macrophages of wild type mice
Contributor(s) Zigmond E, Bernshtein B, Friedlander G, Walker CR, Yona S, Kim K, Brenner O, Krauthgamer R, Varol C, Mueller W, Jung S
Citation(s) 24792913
Submission date Apr 02, 2014
Last update date Mar 04, 2019
Contact name Gilgi Friedlander
Organization name Weizmann Institute of Science
Street address Hertzel st
City Rehovot
ZIP/Postal code 76100
Country Israel
Platforms (1)
GPL6246 [MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version]
Samples (7)
GSM1361638 FACS sorted CX3CR1hi macrophages from the colons of 6 weeks old CX3CR1gfp/+ mice_SJ38
GSM1361639 FACS sorted CX3CR1hi macrophages from the colons of 6 weeks old IL10-/- CX3CR1gfp/+ mice_SJ41
GSM1361640 FACS sorted resident macrophages from the colons of 6 weeks old CX3CR1gfp/+ mice_SJ55
BioProject PRJNA243329

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Supplementary file Size Download File type/resource
GSE56444_RAW.tar 29.4 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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