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| Status |
Public on Dec 05, 2014 |
| Title |
Complex in vivo RNA-seq analysis reveal reprogramming of Yersinia from virulent to persistent mode during infection [microarray I] |
| Platform organism |
Yersinia pseudotuberculosis |
| Sample organism |
Yersinia pseudotuberculosis YPIII |
| Experiment type |
Expression profiling by array
|
| Summary |
Whole transcriptome assessment of the Yersinia pseudotuberculosis strain YPIII grown under aerobic or anaerobic conditions to exponential phase.
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| Overall design |
Y. pseudotuberculosis YPIII was grown at 25°C in LB medium supplemented with 10 g/l glucose and 0.2 M HEPES buffer under aeration or under anaerobic growth conditions (in a nitrogen atmosphere) on a rotary shaker. Total RNA was extracted using SV Total RNA Isolation System (Promega). The samples were treated with RNase-free DNase (Roche Applied Science) and the quality of the RNA was confirmed by the lack of PCR amplification of the hns gene and by using an Agilent 2100 Bioanalyzer.
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| Contributor(s) |
Heroven AK, Beckstette M, Dersch P |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Apr 02, 2014 |
| Last update date |
Dec 06, 2014 |
| Contact name |
Kemal Avican |
| E-mail(s) |
kemal.avican@umu.se
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| Organization name |
Umea University
|
| Department |
Department of Molecular Biology
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| Lab |
Maria Fällman's Lab
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| Street address |
Building 6L
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| City |
Umea |
| ZIP/Postal code |
90187 |
| Country |
Sweden |
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| Platforms (1) |
| GPL15095 |
Agilent-020412 Yersinia pseudotuberculosis YPIII gene expression array |
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| Samples (4)
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| This SubSeries is part of SuperSeries: |
| GSE56477 |
Complex in vivo RNA-seq analysis reveal reprogramming of Yersinia from virulent to persistent mode during infection |
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| Relations |
| BioProject |
PRJNA243388 |
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