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Series GSE56648 Query DataSets for GSE56648
Status Public on Apr 10, 2014
Title Human nuclear Dicer restricts the deleterious accumulation of endogenous double strand RNA
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Genome binding/occupancy profiling by high throughput sequencing
Summary Dicer is a central enzymatic player in RNA interference (RNAi) pathways that acts to regulate gene expression in nearly all eukaryotes. Although the cytoplasmic function of Dicer is well documented in mammals, there is little known about any possible nuclear function. Here we show that Dicer is present in both the nucleus and cytoplasm, but that its nuclear levels are tightly regulated. In its nuclear manifestation Dicer interacts with RNA polymerase II (Pol II) at actively-transcribed gene loci. Loss of Dicer causes the appearance of endogenous dsRNA, leading to induction of the interferon response pathway and consequent cell death. Our results suggest that Pol II-associated Dicer restricts endogenous dsRNA formation from overlapping non-coding RNA transcription units. Failure to do so has catastrophic effects on cell function. Taken together, we present here a micro (mi)RNA independent role for human nuclear Dicer.
Overall design DICER ChIP-seq profile in wt 293 HEK cells, and dsRNA-seq profile in wt and DICER-depleted 293 HEK cells
Contributor(s) White E, Schlackow M, Kamieniarz-Gdula K, Proudfoot N, Gullerova M
Citation(s) 24814348
Submission date Apr 09, 2014
Last update date May 15, 2019
Contact name Kinga Kamieniarz-Gdula
Organization name University of Oxford
Department Sir William Dunn School of Pathology
Street address South Parks Road
City Oxford
ZIP/Postal code OX1 3RE
Country United Kingdom
Platforms (2)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
GPL11154 Illumina HiSeq 2000 (Homo sapiens)
Samples (3)
GSM1366346 dsRNA in wt cells
GSM1366347 dsRNA in DICER kd cells
BioProject PRJNA244157
SRA SRP041057

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Supplementary file Size Download File type/resource
GSE56648_RAW.tar 76.5 Mb (http)(custom) TAR (of BEDGRAPH, TXT, XLSX)
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Raw data are available in SRA
Processed data provided as supplementary file

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