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| Status |
Public on Jan 05, 2015 |
| Title |
Comparison of gene expression of WT and PerM mutant M. tuberculosis H37Rv grown in high and reduced magnesium |
| Platform organism |
Mycobacterium tuberculosis H37Rv |
| Sample organism |
Mycobacterium tuberculosis |
| Experiment type |
Expression profiling by array
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| Summary |
M. tuberculosis membrane protein PerM is required for persistence in chronic mouse infection. In vitro, the perM mutant requires increased magnesium for replication and survival, with elongation and lysis in low-magnesium media. To gain insight into the function of PerM, we compared the transcriptomes of wt and perM mutant Mtb grown in high (2000 μM) and reduced (250 μM) Mg2+ in three independent experiments. Transcriptome analysis of the perM mutant grown in reduced magnesium revealed upregulation of cell division and cell wall biosynthesis genes.
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| Overall design |
Bacteria were grown for 5 days in Sauton’s media containing 250 or 2000 μM MgCl2 and 0.05% Tween 80. Three independent experiments were performed.
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| Contributor(s) |
Ehrt S, Goodsmith N |
| Citation(s) |
25658098 |
| Submission date |
May 03, 2014 |
| Last update date |
Aug 24, 2015 |
| Contact name |
Sabine Ehrt |
| E-mail(s) |
sae2004@med.cornell.edu
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| Organization name |
Weill Cornell Medical College
|
| Department |
Microbiology & Immunology
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| Street address |
413 East 69th Street
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| City |
New York |
| State/province |
New York |
| ZIP/Postal code |
10021 |
| Country |
USA |
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| Platforms (1) |
| GPL16177 |
Agilent-019943 Genotypic Technology custom M tuberculosis h37Rv Whole Genome 8x15k GE Microarray [Gene Name version] |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA246114 |
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