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| Status |
Public on Apr 27, 2015 |
| Title |
Genome-wide maps of primary and processed start-sites of transcripts revealed mechanism controlling in vivo stoichiometry of protein complex in bacteria |
| Organism |
Ruminiclostridium cellulolyticum |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Here a differential mRNA-sequencing (dRNA-Seq) strategy was employed to distinguish transcriptional start-sites (TSs) and post-transcriptional processed sites (PSs) of transcripts in the cellulolytic mesophile Clostridium cellulolyticum. PS-harboring genes usually encode heteromultimeric complexes such as flagella, ribosomes, F-type ATPases, and cellulosomes. Intriguingly, PSs located in intergenic reigons (iPSs) were appear to be associated with a particular set of operons that expressed as a complex ratio, in which stem-loops were often found at upstream and/or downstream of highly-expressed processed transcripts; among operons, the folding free energy (deltaG) of the stem-loops is negatively correlated with polarity in transcript level of the operon.
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| |
|
| Overall design |
mRNA profiles of C. cellulolyticum H10 grown under three carbon sources in two RNA treatments were generated by deep sequencing, in duplicate, using Illumina HiSeq 2000
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| |
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| Contributor(s) |
Xu C, Xu J |
| Citation(s) |
25908225, 29467821 |
| |
| Submission date |
May 13, 2014 |
| Last update date |
May 31, 2019 |
| Contact name |
Jian Xu |
| E-mail(s) |
xujian@qibebt.ac.cn
|
| Organization name |
Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences
|
| Lab |
Jian lab
|
| Street address |
No.189 Songling Road, Laoshan District
|
| City |
Qingdao |
| State/province |
Shandong |
| ZIP/Postal code |
266101 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL18692 |
Illumina HiSeq 2000 (Clostridium cellulolyticum) |
|
| Samples (12)
|
|
| Relations |
| BioProject |
PRJNA247408 |
| SRA |
SRP041966 |
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