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Series GSE57820 Query DataSets for GSE57820
Status Public on Apr 21, 2015
Title The effect of miRNA-135b overexpression on the gene expression profile of LNCaP cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary MicroRNAs (miRNAs) regulate a wide range of cellular signaling pathways and biological processes in both physiological and pathological states such as cancer. We have previously identified miR-135b as a direct regulator of androgen receptor (AR) protein level in prostate cancer (PCa). We wanted to further explore the relationship of miR-135b to hormonal receptors, particularly estrogen receptor α (ERα). Here we show that miR-135b expression inversely correlates with ER protein in two independent breast cancer (BCa) patient cohorts (101 and 1302 samples) and with AR protein in 47 PCa patient samples. We identify ERα as a novel miR-135b target by demonstrating miR-135b binding to the 3’UTR of the ERα and decreased ERα protein and mRNA level in breast cancer cells upon miR-135b overexpression. miR-135b inhibits proliferation of hormone receptor positive cancer cell lines as shown by overexpression in ERα-positive BCa cells (MCF-7) and AR-positive PCa cells (LNCaP, 22Rv1) when grown in 2D. To identify other genes regulated by miR-135b we performed gene expression studies and found a potential link to the hypoxia-inducible factor-1α (HIF1α) pathway. We show that miR-135b influences the protein level of the inhibitor for hypoxia-inducible factor-1 (HIF1AN), which also demonstrated an inverse correlation with miR-135b in a cohort of breast tumor samples. Taken together, our study demonstrates that miR-135b regulates ERα, AR and HIF1AN protein levels and proliferation in ERα -positive breast and AR-positive-prostate cancer cells.
Overall design LNCaP cells were transfected with Ambion pre-miR™ construct for miR-135b or with pre-miR negative control #1 (scrambled pre-miR, Scr) at 20 nM, and incubated for 12h, 24h or 36h, in two biological repeats (B1 and B2)
Contributor(s) Aakula A, Leivonen S, Hintsanen P, Aittokallio T, Ceder Y, Børresen-Dale A, Perälä M, Östling P, Kallioniemi O
Citation(s) 25907805
Submission date May 20, 2014
Last update date Aug 13, 2018
Contact name Anna Aakula
Organization name Institute for Molecular Medicine Finland, FIMM
Street address Tukholmankatu 8
ZIP/Postal code 00290
Country Finland
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (12)
GSM1394594 LNCaP_miR-135b_12h_B1
GSM1394595 LNCaP_miR-135b_12h_B2
GSM1394596 LNCaP_miR-135b_24h_B1
BioProject PRJNA248178

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE57820_RAW.tar 26.2 Mb (http)(custom) TAR
GSE57820_non_normalized.txt.gz 3.6 Mb (ftp)(http) TXT
Processed data included within Sample table

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