|
| Status |
Public on May 23, 2014 |
| Title |
Using C. intestinalis to study Islet in ascidian palp morphogenesis |
| Organism |
Ciona intestinalis |
| Experiment type |
Expression profiling by array
|
| Summary |
In Ciona intestinalis, the palps consist of three conical protrusions within a field of thickened epithelium that form late in embryogenesis as tailbuds mature into larvae. The palp protrusions express the LIM-homeodomain transcription factor Islet. Protrusion occurs through differential cell elongation, likely mediated by Islet, as we find that ectopic expression of Islet is sufficient to promote cell elongation. FGF signaling is required for both Islet expression and palp morphogenesis. Importantly, we show that Islet expression can rescue the palp-deficient phenotype that results from inhibition of FGF signaling. We conclude that Islet is a key regulatory factor governing morphogenesis of the palps. It is conceivable that Islet is also essential for the cellular morphogenesis of placode-derived sensory neurons in vertebrates.
Islet expression can rescue the palp-deficient phenotype that results from inhibition of FGF signaling, and conclude that Islet is a key regulatory factor governing morphogenesis of the palps.
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| |
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| Overall design |
Three biological replicates were analyzed for both sample types (Fox positive and Negative).
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| |
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| Contributor(s) |
Wagner E, Stolfi A, Choi YG, Levine M |
| Citation(s) |
24993943 |
| |
| Submission date |
May 22, 2014 |
| Last update date |
Sep 11, 2014 |
| Contact name |
Justin Choi |
| E-mail(s) |
jygchoi@berkeley.edu
|
| Phone |
510-642-1165
|
| Organization name |
UC Berkeley
|
| Department |
Functional Genomics lab
|
| Street address |
255 Life Sciecnces Addition
|
| City |
Berkeley |
| State/province |
CA |
| ZIP/Postal code |
94720 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL15657 |
[CINT06a520380F] Affymetrix Ciona intestinalis custom-designed array |
|
| Samples (6)
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| Relations |
| BioProject |
PRJNA248413 |
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