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| Status |
Public on Dec 31, 2016 |
| Title |
miRNAs involed in lactation of cow mammary gland |
| Organism |
Bos taurus |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
Expression profiling by high throughput sequencing
|
| Summary |
We investigated miRNA expression in Holstein dairy cow of mammary gland with different producing quality milk using high-throughput sequence and qRT-PCR techniques. miRNA libraries were constructed from mammary gland tissues taken from a high producing quality milk and a low producing quality milk Holstein dairy cow, the small RNA digitalization analysis based on HiSeq high-throughput sequencing takes the SBS-sequencing by synthesis.The libraries included 4732 miRNAs. A total of 124 miRNAs in the high producing quality milk mammary gland showed significant differences in expression compared to low producing quality milk mammary gland (P<0.05).
Conclusion: Our study provides a broad view of the bovine mammary gland small RNA expression profile characteristics. Differences in types and expression levels of miRNAs were observed between high producing quality milk and a low producing quality milk Holstein dairy cow
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| |
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| Overall design |
Examination of 3 group of different producing quality milk mammary gland of Holstein dairy cow,each group has 3 sample
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| |
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| Contributor(s) |
Wang C, Wang J, Li D, Li Q |
| Citation(s) |
24987964 |
| |
| Submission date |
May 27, 2014 |
| Last update date |
Aug 01, 2019 |
| Contact name |
Chunmei wang |
| E-mail(s) |
wangcm-1@163.com
|
| Phone |
08613936507139
|
| Organization name |
Northeast Agricultural University
|
| Street address |
59 MuCai Street
|
| City |
Haerbin |
| ZIP/Postal code |
150030 |
| Country |
China |
| |
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| Platforms (1) |
| GPL15749 |
Illumina HiSeq 2000 (Bos taurus) |
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| Samples (3) |
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| Relations |
| BioProject |
PRJNA248657 |
| SRA |
SRP042291 |
