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| Status |
Public on Aug 12, 2014 |
| Title |
Transcriptional response to stress in serum deprived mouse fibroblasts in the presence of MSK1/2 inhibitor. |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We have employed gene expression profiling in order to identify targets of transcriptional response to stress in resting mouse Swiss 3T3 fibroblasts, either untreated (control) or treated with anisomycin for 3 or 6 hours to induce the p38/MAP kinase pathway. In order determine transcriptional effects dependent on MSK1/2 kinase activity, H89 inhibitor was used in the study.
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| Overall design |
Serum starved (72 h 0.2% FCS) mouse 3T3 cells were treated with anisomycin (188.5 nM) for 3 h or 6h (in duplicates) either with or without 15-min pre-treatment with MSK1/2 inhibitor H89 (10 uM). Untreated, serum-starved cells were used as a control. RNA was collected and gene expression profiling using strand-specific RNA-seq was performed.
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| Contributor(s) |
Sawicka A, Seiser C |
| Citation(s) |
25135956 |
| |
| Submission date |
Jun 23, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Anna Sawicka |
| E-mail(s) |
anna.sawicka@mpibpc.mpg.de
|
| Organization name |
Max Planck Institure for Biophysical Chemistry
|
| Department |
Dep. of Molecular Biology
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| Lab |
Cramer
|
| Street address |
Am Fassberg 11
|
| City |
Goettingen |
| ZIP/Postal code |
37077 |
| Country |
Germany |
| |
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| Platforms (1) |
| GPL13112 |
Illumina HiSeq 2000 (Mus musculus) |
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| Samples (14)
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| This SubSeries is part of SuperSeries: |
| GSE55784 |
Genome-wide analysis of stress response in mouse fibroblasts |
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| Relations |
| BioProject |
PRJNA253332 |
| SRA |
SRP043512 |
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