|
Status |
Public on Jun 13, 2015 |
Title |
Critical role of histone demethylase Jmjd3 in the regulation of CD4+ T cell differentiation |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
Summary |
Epigenetic factors have been implicated in the regulation of CD4(+) T-cell differentiation. Jmjd3 plays a role in many biological processes, but its in vivo function in T-cell differentiation remains unknown. Here we report that Jmjd3 ablation promotes CD4(+) T-cell differentiation into Th2 and Th17 cells in the small intestine and colon, and inhibits T-cell differentiation into Th1 cells under different cytokine-polarizing conditions and in a Th1-dependent colitis model. Jmjd3 deficiency also restrains the plasticity of the conversion of Th2, Th17 or Treg cells to Th1 cells. The skewing of T-cell differentiation is concomitant with changes in the expression of key transcription factors and cytokines. H3K27me3 and H3K4me3 levels in Jmjd3-deficient cells are correlated with altered gene expression through interactions with specific transcription factors. Our results identify Jmjd3 as an epigenetic factor in T-cell differentiation via changes in histone methylation and target gene expression.
|
|
|
Overall design |
ChIP-seq of histone modification marks H3K4me3 and H3K27me3 in WT and JMJD3 cKO mouse CD4+ T-cells
|
|
|
Contributor(s) |
Chepelev I |
Citation(s) |
25531312 |
|
Submission date |
Jun 24, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Iouri Chepelev |
E-mail(s) |
ichepelev@gmail.com
|
Organization name |
US Department of Veterans Affairs Medical Center
|
Street address |
3200 Vine St
|
City |
Cincinnati |
State/province |
OH |
ZIP/Postal code |
45220 |
Country |
USA |
|
|
Platforms (1) |
GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
|
Samples (8)
|
|
Relations |
BioProject |
PRJNA253470 |
SRA |
SRP043531 |