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| Status |
Public on Jul 01, 2015 |
| Title |
Haemophilus influenzae 86-028NP |
| Organism |
Haemophilus influenzae |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Transcriptome analysis of NTHi 86-028NPrpsL, NTHi 86-028NPrpsL∆fur, and NTHi 86-028NPrpsL∆fur(pT-fur) strains
Nontypeable Haemophilus influenzae (NTHi) is a commensal microorganism of the normal human nasopharyngeal flora, yet also an opportunistic pathogen of the upper and lower respiratory tracts. Changes in gene expression patterns in response to host microenvironments are likely critical for survival. One such system of gene regulation is the ability to carefully regulate iron uptake. A central regulatory system that controls iron uptake, mediated by the ferric uptake regulator Fur, is present in multiple bacteria, including NTHi. To understand the regulation of iron homeostasis in NTHi, fur was deleted in the NTHi strain 86-028NPrpsL. Using RNA-Seq, we identified both protein-encoding and small RNA genes whose expression was repressed or activated by Fur. Overall design: These data comprise transcriptional anaylses of an rpsL mutant of 86-028NP, an isogenic fur mutant of 86-028NPrpsL and a complemented fur mutant strain. All strains were grown in defined medium containing 10 µg/ml human hemoglobin to mid-log phase. Cells were then harvested and RNA extracted. A total of three biological replicates were generated for these analyses.
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| Overall design |
Analysis of transcriptomes using the Illumina HiSeq 2000 of three strains of nontypeable Haemophilus influenzae which include NTHi 86-028NPrpsL, NTHi 86-028NPrpsL∆fur, and NTHi 86-028NPrpsL∆fur(pT-fur) strains. For each strain three biological replicates were analyzed
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| Contributor(s) |
Harrison A, Munson RS Jr, Santana EA |
| Citation(s) |
25157846 |
| BioProject |
PRJNA248129 |
| |
| Submission date |
Jun 27, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Alistair Harrison |
| Organization name |
The Research Institute at Nationwide Children's Hospital
|
| Department |
Center for Microbial Pathogenesis
|
| Street address |
700 Children's Drive
|
| City |
Columbus |
| State/province |
OH |
| ZIP/Postal code |
43205 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL18879 |
Illumina HiSeq 2000 (Haemophilus influenzae) |
|
| Samples (9)
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| Relations |
| SRA |
SRP042197 |
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