|
| Status |
Public on Dec 01, 2014 |
| Title |
Transcriptional profiling of E. coli cultures exposed to green or red CdTe Quantum Dots 50 µg/ml |
| Platform organism |
Escherichia coli |
| Sample organism |
Escherichia coli K-12 |
| Experiment type |
Expression profiling by array
|
| Summary |
E. coli cultures were exposed to green or red CdTe Quantum Dots 50 µg/ml during 15 min. Total RNA was extracted and cDNA labeled probes were generated by reverse transcription using Alexa 555 and Alexa 647 fluorophores. These probes were used to hybridize genomic slides containing genomic arrays to determine global transcriptional changes.
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| |
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| Overall design |
Two-conditions experiment, Quantum Dots treated cells vs. Untreated control cells. Biological replicates: 3 control, 3 toxicants exposed cells, independently grown and harvested. One replicate per array
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| |
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| Contributor(s) |
Loyola DE |
| Citation(s) |
25496196 |
| |
| Submission date |
Jun 30, 2014 |
| Last update date |
Oct 17, 2019 |
| Contact name |
David Enrique Loyola |
| E-mail(s) |
dloyolac@gmail.com
|
| Phone |
+56-9-74305392
|
| Organization name |
OMICS Solutions, Centro Nacional de Genómica y Bioinformática
|
| Street address |
Avenida Libertador Bernardo O'Higgins 340
|
| City |
Santiago |
| State/province |
Santiago |
| ZIP/Postal code |
8331150 |
| Country |
Chile |
| |
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| Platforms (1) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA253924 |
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