|
| Status |
Public on Sep 30, 2014 |
| Title |
miRNA and consolidation of cued fear memory |
| Platform organisms |
Homo sapiens; Mus musculus; Rattus norvegicus; Human alphaherpesvirus 1; Human alphaherpesvirus 2; Human betaherpesvirus 5; Murid betaherpesvirus 1; human gammaherpesvirus 4; JC polyomavirus; Human immunodeficiency virus 1; Murid gammaherpesvirus 4; Human gammaherpesvirus 8; Merkel cell polyomavirus; Mus musculus cytomegalovirus 2; Betapolyomavirus hominis; Betapolyomavirus macacae |
| Sample organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
miRNA profiling was carried out using the miRCURY LNA™ microRNA Array (6th gen - hsa, mmu & rno)
miRNA were profiled in amygdala brain tissue obtained from adult mice 30 mins after auditory fear conditioning and expression levels compared to tissue obtained from Home cage controls
|
| |
|
| Overall design |
Adult male mice were fear conditioned using tone-shock pairings and brains were harvested 30 mins later. The brains of Home Cage controls and Fear Conditioned animals (n = 4/group) were then punched to collect amygdala tissue. miRNA were extracted using the Qiagen miRNeasy Kit, and then shipped to Exiqon. Exiqon performed labeling, hybridization and data analysis after use of the miRCURY LNA™ microRNA Array (6th gen - hsa, mmu & rno). https://www.exiqon.com/ls/Documents/Scientific/miRCURY-LNA-microRNA-Array-6th-gen-hsa-mmu-rno-manual.pdf
|
| |
|
| Contributor(s) |
Dias BG, Ressler KJ |
| Citation(s) |
25123309 |
| |
| Submission date |
Jul 03, 2014 |
| Last update date |
May 10, 2016 |
| Contact name |
Brian George Dias |
| Organization name |
Emory University
|
| Department |
Psychiatry and Behavioral Sciences
|
| Street address |
954 Gatewood Rd NE
|
| City |
Atlanta |
| State/province |
GA |
| ZIP/Postal code |
30324 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11434 |
miRCURY LNA microRNA Array, 6th generation - hsa, mmu & rno |
|
| Samples (8)
|
|
| Relations |
| BioProject |
PRJNA254328 |
Less...
More...