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Series GSE59227 Query DataSets for GSE59227
Status Public on Feb 29, 2016
Title A Molecular Portrait of the replication stress response defects via Genome-wide Transcriptome Profiling
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Defects in replication stress response (RSR) allow the survival and proliferation of genomically unstable cells, ultimately leading to tumorigenesis. Therefore, the RSR status can potentially serve as a powerful indicator to predict the possibility of early tumorigenesis. Here, we identified an RSR defects (RSRD) gene signature that robustly predicted RSR status. For the clinical benefit, our identification of RSRD gene signature gives the possibility to assess the risk of early tumorigenesis in the precancerous patients.
 
Overall design Various shRNAs that target genes involved in replication stress response were transfected in MCF-10A non-transformed breast cell lines after oncogenic cyclin E overexpressed. All MCF-10A variants were seeded 200000 at 10 cm plate. Cells were harvested after 48 hours culturing and used for gene expression profiling.
 
Contributor(s) Lin CC, Dai H, Lin S
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Submission date Jul 09, 2014
Last update date Aug 13, 2018
Contact name Chun-Jen Curtis Lin
E-mail(s) cclin@mdanderson.org
Organization name University of Texas MD Anderson Cancer Center
Department Systems Biology
Lab Dr. Shiaw-Yih Lin
Street address 7345 Fannin Street
City Houston
State/province Texas
ZIP/Postal code 77054
Country USA
 
Platforms (1)
GPL10558 Illumina HumanHT-12 V4.0 expression beadchip
Samples (30)
GSM1430964 MCF-10A shControl_cyclin E overexpression set 1 replicate 1
GSM1430965 MCF-10A shControl_cyclin E overexpression set 1 replicate 2
GSM1430966 MCF-10A shControl_cyclin E overexpression set 1 replicate 3
Relations
BioProject PRJNA254773

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE59227_RAW.tar 26.2 Mb (http)(custom) TAR
GSE59227_non-normalized_data.txt.gz 8.3 Mb (ftp)(http) TXT
Processed data included within Sample table
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