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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Mar 23, 2015 |
| Title |
Regulation of gene expression by loss-of-YAP/TAZ in MDA-MB-231 breast cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
YAP1 (Yes-associated protein 1) and TAZ (transcriptional coactivator with PDZ-binding motif, or WWTR1) are nucleo-cytoplasmic shuttling proteins that can function in the nucleus as transcriptional coactivators. Their role in regulating gene transcription has been so far mainly investigated by overexpressing YAP1 or TAZ, while here we sought to determine which genes are regulated by endogenous levels of YAP/TAZ. To this end, we compared MCF10A cells transfected with a control non-targeting siRNA to cells transfected with two independent mixes of siRNA targeting both YAP and TAZ.
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| Overall design |
MDA-MB-231 cells growing at low confluence in standard growth conditions were transfected with the indicated siRNAs (Dupont et al., Nature 2011). The following day, growth medium was renewed and cells were incubated for 24 more hours, and then harvested for total RNA extraction. 4 independent biological replicates were plated, transfected ans harvested in parallel for each siRNA.
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| Contributor(s) |
Dupont S, Bicciato S |
| Citation(s) |
25796446 |
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| Submission date |
Jul 09, 2014 |
| Last update date |
Mar 25, 2019 |
| Contact name |
Silvio Bicciato |
| E-mail(s) |
silvio.bicciato@unipd.it
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| Phone |
+39-049-827-6108
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| Organization name |
University of Padova
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| Department |
Molecular Medicine
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| Street address |
via U. Bassi 59/b
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| City |
Padova |
| ZIP/Postal code |
35131 |
| Country |
Italy |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (12)
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| GSM1431022 |
MDA-MB-231 cells siControl, biological replica A |
| GSM1431023 |
MDA-MB-231 cells siControl, biological replica B |
| GSM1431024 |
MDA-MB-231 cells siControl, biological replica C |
| GSM1431025 |
MDA-MB-231 cells siControl, biological replica D |
| GSM1431026 |
MDA-MB-231 cells siYAP/TAZ#1, biological replica A |
| GSM1431027 |
MDA-MB-231 cells siYAP/TAZ#1, biological replica B |
| GSM1431028 |
MDA-MB-231 cells siYAP/TAZ#1, biological replica C |
| GSM1431029 |
MDA-MB-231 cells siYAP/TAZ#1, biological replica D |
| GSM1431030 |
MDA-MB-231 cells siYAP/TAZ#2, biological replica A |
| GSM1431031 |
MDA-MB-231 cells siYAP/TAZ#2, biological replica B |
| GSM1431032 |
MDA-MB-231 cells siYAP/TAZ#2, biological replica C |
| GSM1431033 |
MDA-MB-231 cells siYAP/TAZ#2, biological replica D |
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| This SubSeries is part of SuperSeries: |
| GSE59232 |
Control of YAP/TAZ by glucose metabolism |
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| Relations |
| BioProject |
PRJNA254774 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE59230_RAW.tar |
56.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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