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| Status |
Public on Jan 01, 2018 |
| Title |
Generating IGF1 signatures in human melanoma cell lines by activation of IGF1 or ihibition of IGF1R |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To generate IGF1 signatures in human melanoma cell lines. Recombinant human IGF1 (rhIGF1) was added to WM35, known to be responsive to rhIGF1, whereas constitutive IGF1 signaling in Mel-624 was abrogated by siRNA directed against the IGF1 receptor (siIGF1R). Experiments were performed in triplicate in normoxia (O2 concentration, 21%) and hypoxia (1% O2), because hypoxia has been suspected to play an adverse role in melanoma. After generating GEP data, we created signatures of genes significantly (p ≤0.0001, q ≤0.05) and consistently upregulated in rhIGF1-treated WM35 cells, or similarly downregulated in siIGF1R-treated Mel-624 cells, as compared to control cells at the same O2 concentration.
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| Overall design |
To find out whether IGF1 signatures generated in human melanoma cell lines are predictive of outcome in melanoma patients, we generated IGF1 signatures in two cell lines WM35 and Mel-624. The expression of IGF1 signatures were then tested by using GSSA (Gene Signature Survival Analysis) method to find out correlation with clinical outcome of melanoma patients.
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| Contributor(s) |
Ma W, Wang Z, Davis RE, Tsai KY |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jul 11, 2014 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Wencai Ma |
| Organization name |
MD Anderson Cancer Center
|
| Street address |
7455 Fannin st
|
| City |
Houston |
| ZIP/Postal code |
77054 |
| Country |
USA |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (24)
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| This SubSeries is part of SuperSeries: |
| GSE59409 |
Correlating Gene Signatures with Outcome to find Features and Drugs Relevant to Cancer |
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| Relations |
| BioProject |
PRJNA255116 |
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