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| Status |
Public on Mar 25, 2007 |
| Title |
Wheat expression level polymorphism study parental genotypes 2 biological reps from SB location |
| Organism |
Triticum aestivum |
| Experiment type |
Expression profiling by array
|
| Summary |
The use of statistical tools established for the genetic analysis of quantitative traits can be applied to gene expression data. Quantitative trait loci (QTL) analysis can associate expression of genes or groups of genes with particular genomic regions and thereby identify regions that play a role in the regulation of gene expression. A segregating population of 41 doubled haploid (DH) lines from the hard red spring wheat cross RL4452 x AC Domain was used. This population had previously been mapped with microsatellites and includes a full QTL analysis for agronomic and seed quality traits. Expression analysis from 5 day post anthesis developing seed was conducted on 36 of the 41 DH lines using the Affymetrix wheat array. Expression analysis of developing seeds from field grown material in location 2 identified 10,280 sequences represented by Affymetrix probe sets whose expression varied significantly between genotypes of the population. Of these 1,455 were identified in the point location as well. A sub-set of 542 transcripts were identified that each mapped to a single chromosome interval illustrating that major expression QTLs can be found in wheat. Genomic regions corresponding to multiple expression QTLs were identified that were coincident with previous identified seed quality trait QTL. These regions may be important regulatory regions governing economically important traits. Comparison of expression mapping data with physical mapping for a sub-set of sequences showed that both cis and trans acting expression QTLs were present.
Keywords: genetic differences, expression QTL
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| Overall design |
Parental genotypes of mapping population RL4452 x AC Domain with 2 biological replicates per genotype. Grown in the field in a lattice design at SB location with the 41 DH progeny lines.
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| |
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| Contributor(s) |
Jordan M, Somers D |
| Citation(s) |
17388792 |
| |
| Submission date |
Sep 28, 2006 |
| Last update date |
Dec 04, 2018 |
| Contact name |
Mark Jordan |
| E-mail(s) |
mcjordan@agr.gc.ca
|
| Organization name |
Agriculture and Agri-Food Canada
|
| Department |
Cereal research Centre
|
| Street address |
195 Dafoe Rd.
|
| City |
Winnipeg |
| State/province |
Manitoba |
| ZIP/Postal code |
R3T 2M9 |
| Country |
Canada |
| |
|
| Platforms (1) |
| GPL3802 |
[wheat] Affymetrix Wheat Genome Array |
|
| Samples (4)
|
| GSM138136 |
Genotype Domain developing seed at 5 days after anthesis, biological rep1 location SB |
| GSM138137 |
Genotype Domain developing seed at 5 days after anthesis, biological rep3 location SB |
| GSM138144 |
Genotype RL4452 developing seed at 5 days after anthesis, biological rep1 location SB |
| GSM138145 |
Genotype RL4452 developing seed at 5 days after anthesis, biological rep3 location SB |
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| This SubSeries is part of SuperSeries: |
| GSE5942 |
Wheat expression level polymorphism study parentals and progenies from SB location |
|
| Relations |
| BioProject |
PRJNA104425 |
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