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Status |
Public on Oct 01, 2014 |
Title |
Anaerobic Degradation of Butyrate By the Hyperthermophilic Archaeon, Ferroglobus placidus |
Platform organism |
Ferroglobus placidus DSM 10642 |
Sample organism |
Ferroglobus placidus |
Experiment type |
Expression profiling by array
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Summary |
Comparison of acetate- to butyrate-grown F. placidus cells to identify genes that are potentially involved in fatty acid degradation by this unique hypertherophilic archaeon.
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Overall design |
A four chip study using total RNA recovered from three separate cultures of Ferroglobus placidus DSM 10642 grown with 1 mM butyrate (experimental condition) and three separate cultures of Ferroglobus placidus DSM 10642 grown on 10 mM acetate (control condition). Each chip measures the expression level of 2613 genes from Ferroglobus placidus DSM 10642 with nine 45-60-mer probe pairs (PM/MM) per gene, with three-fold technical redundancy.
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Contributor(s) |
Holmes D, Risso C, Smith J, Lovley D |
Citation missing |
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Submission date |
Jul 16, 2014 |
Last update date |
Oct 01, 2014 |
Contact name |
Dawn E Holmes |
E-mail(s) |
dholmes@microbio.umass.edu
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Phone |
4135772439
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Organization name |
University of Massachusetts Amherst
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Department |
Microbiology
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Lab |
Lovley
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Street address |
639 N Pleasant St Morrill IVN 422C
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City |
Amherst |
State/province |
Massachusetts |
ZIP/Postal code |
01003 |
Country |
USA |
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Platforms (1) |
GPL18954 |
NimbleGen Ferroglobus placidus strain DSM 10642 v1.0 (07/16/2014) |
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Samples (6)
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Relations |
BioProject |
PRJNA255391 |