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| Status |
Public on May 12, 2015 |
| Title |
The transcriptome of TRPV1-positive sensory neurons revealed by subgroup-elimination transcriptomics |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by array
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| Summary |
We developed an approach to rapidly eliminate the subgroup of sensory neurons expressing the heat-gated cation channel TRPV1 from dissociated rat sensory ganglia using agonist treatment followed by density centrifugation. To identify transcripts predomintly expressed in TRPV1-positive neurons, we compared the transcriptome of all cells within sensory ganglia versus all cells without TRPV1 expressing neurons using RNA-Seq.
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| Overall design |
Four replicate experiments with RNA from DRG neurons of one rat per experiment were performed. Dissociated neurons were split up in three parts, treated with solvent DMSO (0.1%), casaicin (10 µM), or RTX (100 nM) for 30 min followed by gradient centrifugation. RNA was extracted from the remaining pellet containing either all cells or all cells without TRPV1-positive neurons.
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| Contributor(s) |
Isensee J, Wenzel C, Buschow R, Weissmann R, Andres C, Kuss AW, Hucho T |
| Citation(s) |
25551770 |
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| Submission date |
Jul 24, 2014 |
| Last update date |
May 13, 2015 |
| Contact name |
Joerg Isensee |
| E-mail(s) |
joerg.isensee@uk-koeln.de
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| Phone |
+4922147887591
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| Organization name |
University Hospital of Cologne
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| Department |
Department of Anesthesiology and Intensive Care Medicine
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| Lab |
Experimental Anesthesiology and Pain Research
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| Street address |
Robert Koch Str. 10
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| City |
Cologne |
| ZIP/Postal code |
50931 |
| Country |
Germany |
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| Platforms (1) |
| GPL6101 |
Illumina ratRef-12 v1.0 expression beadchip |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA256086 |
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