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| Status |
Public on Nov 26, 2006 |
| Title |
Interdependence between DNA methylation and transcription |
| Organism |
Arabidopsis thaliana |
| Experiment type |
Expression profiling by genome tiling array
Genome binding/occupancy profiling by genome tiling array
Methylation profiling by array
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| Summary |
Cytosine methylation, a common form of DNA modification that antagonizes transcriptional initiation, is found at transposons and repeats in vertebrates, plants, and fungi. Here we have mapped DNA methylation in the entire Arabidopsis thaliana genome at high resolution. DNA methylation covers transposons and is present within about 20% of Arabidopsis genes. Methylation within genes is conspicuously biased away from gene ends, suggesting a dependence on RNA polymerase transit. Genic methylation is strongly influenced by transcription: moderately transcribed genes are most likely to be methylated, while genes at either extreme are least likely. Small RNAs are enriched at methylated genes, which suggests that aberrant transcription triggers genic methylation. Transcription is in turn influenced by methylation: short methylated genes are poorly expressed, and loss of methylation in the body of a gene leads to enhanced transcription. Our results indicate that genic transcription and DNA methylation are closely interwoven processes.
Keywords: whole genome profiling, DNA methylation, met1-6 expression
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| Overall design |
All experiments were done using two channels per chip. Two microarray experiments compared immunoprecipitated, methylated DNA to control genomic DNA. A third microarray experiment used a methylation sensitive restriction enzyme (HpyCH4 IV) to determine regions of differential DNA methylation in met1-6 mutant plants. Two microarray experiments for expression analysis of met1-6 mutants were done using biological replicates. Oligo (dT) primed cDNA from mutant plants was compared to oligo (dT) primed cDNA from wild type plants.
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| Contributor(s) |
Zilberman D, Gehring M, Tran RK, Ballinger T, Henikoff S |
| Citation(s) |
17128275 |
| |
| Submission date |
Oct 05, 2006 |
| Last update date |
Aug 30, 2012 |
| Contact name |
Jorja Henikoff |
| E-mail(s) |
jorja@fhcrc.org
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| Phone |
206-667-4850
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| Organization name |
Fred Hutchinson Cancer Research Center
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| Department |
Basic Sciences
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| Lab |
Henikoff
|
| Street address |
1100 Fairview AV N, A1-162
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| City |
Seattle |
| State/province |
WA |
| ZIP/Postal code |
98109-1024 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL3371 |
FHCRC Arabidopsis Tiling Array |
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| Samples (5)
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| Relations |
| BioProject |
PRJNA97449 |
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