GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE59836 Query DataSets for GSE59836
Status Public on Nov 17, 2014
Title Recombination initiation maps of individual human genomes
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary DNA double-strand breaks (DSBs) are introduced in meiosis to initiate recombination and to generate crossovers, the reciprocal exchanges of genetic material between parental chromosomes. Here we present the first high-resolution map of meiotic DSBs in individual human genomes. Comparing DSB maps between individuals shows that along with DNA binding by PRDM9, additional factors dictate the efficiency of DSB formation. Furthermore, we find that in human males, the frequency of DSB formation is the primary determinant of crossover rate. Patterns of sequence polymorphisms around meiotic DSB hotspots provide evidence for both GC-biased gene conversion and for a mutagenic role of DSB repair and/or recombination. Finally, we provide compelling evidence that the aberrant repair of meiotic DSBs is a driver of human genomic disorders.
Overall design Detection of meiotic double strand breaks in testis of several human male individuals.
Contributor(s) Pratto F, Brick K, Khil P, Smagulova F, Petukhova G, Camerini-Otero R
Citation(s) 25395542
Submission date Jul 28, 2014
Last update date Feb 09, 2021
Contact name Kevin Brick
Organization name NIDDK
Department GBB
Street address 5/205 Memorial Drive
City Bethesda
State/province MD
ZIP/Postal code 20892
Country USA
Platforms (1)
GPL16791 Illumina HiSeq 2500 (Homo sapiens)
Samples (15)
GSM1447325 AA1 individual Anti-DMC1
GSM1447326 AA1 individual Input DNA
GSM1447327 AA1 individual genomic DNA
BioProject PRJNA256331
SRA SRP044944

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE59836_Peak_data_Supplementary_File_1.txt.gz 1021.3 Kb (ftp)(http) TXT
GSE59836_RAW.tar 3.8 Gb (http)(custom) TAR (of BED)
GSE59836_VariantCalls_AB1_AA1_AA2_Annotated.vcf.gz 484.8 Mb (ftp)(http) VCF
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap