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Series GSE59837 Query DataSets for GSE59837
Status Public on Jul 28, 2014
Title Differential Responses of Plasmacytoid Dendritic Cells to Influenza and Distinct Viral Pathogens
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Plasmacytoid dendritic cells (pDCs) are key components of the innate immune response that are capable of synthesizing and rapidly releasing vast amounts of type I interferons (IFNs), particularly IFN-alpha. Here we investigated whether pDCs, often regarded as a mere source of IFN, discriminate between various functionally discrete stimuli and to what extent this reflects differences in pDC responses other than IFN-alpha release. To examine the ability of pDCs to differentially respond to various doses of intact and infectious HIV, hepatitis C virus, and H1N1 influenza virus, whole-genome gene expression analysis, enzyme-linked immunosorbent assays, and flow cytometry were used to investigate pDC responses at the transcriptional, protein, and cellular levels. Our data demonstrate that pDCs respond differentially to various viral stimuli with significant changes in gene expression, including those involved in pDC activation, migration, viral endocytosis, survival, or apoptosis. In some cases, the expression of these genes was induced even at levels comparable to that of IFN-alpha. Interestingly, we also found that depending on the viral entity and the viral titer used for stimulation, induction of IFN-alpha gene expression and the actual release of IFN-alpha are not necessarily temporally coordinated. In addition, our data suggest that high-titer influenza A (H1N1) virus infection can stimulate rapid pDC apoptosis.
 
Overall design pDCs from 3 separate donors (biological replicates) were stimulated with PBS (control 1) (n=3), conditioned medium (control 2) (n=3), or HCV JFH-1 at 1.00x10^4 (n=3), 1.00x10^5 (n=3), or 1.00x10^6 (n=3) RNA copies/ml. pDCs from 3 separate donors (biological replicates) were stimulated with PBS (control) (n=3) or influenza virus A/PR/8/34 (H1N1; Charles River Laboratories) at 4 (n=3), 32 (n=3), 256 (n=3), or 2,048 (n=3) HAU/ml. pDCs from 3 separate donors (biological replicates) were stimulated with PBS (control) (n=3) or HIV-1 IIIB (Advanced Biotechnologies) at 1.00x10^2 (n=3), 1.00x10^3 (n=3), 1.00x10^4 (n=3), or 1.00x10^5 (n=3) 50% tissue culture infective doses (TCID50)/ml. pDCs from 10 separate donors (biological replicates) were treated with PBS (control) (n=10), HCV at 1.00x10^6 RNA copies/ml (n=10), HIV-1 BaL at 1.00x10^5 TCID50/ml (Advanced Biotechnologies) (n=10), HIV-1 IIIB at 1.00x10^5 TCID50/ml (Advanced Biotechnologies) (n=10), or influenza virus A/PR/8/34 (H1N1; Charles River Laboratories) at 4 HAU/ml (n=10).
 
Citation(s) 25008918
Submission date Jul 28, 2014
Last update date Jul 28, 2014
Contact name Francesco Maria Marincola
E-mail(s) fmarincola@sidra.org
Phone 301-793-8210
Organization name Sidra Medical and Research Center
Street address Al Nasr Tower, AL Corniche Street, PO Box 26999
City Doha
ZIP/Postal code PO Box 26999
Country Qatar
 
Platforms (1)
GPL7088 CCDTM Hs_CCDTM36k - version 1
Samples (95)
GSM1447230 H1N1 - Donor X - PBS Control - mAdbID:107160
GSM1447231 H1N1 - Donor X - 4 HAU/ml - mAdbID:107161
GSM1447232 H1N1 - Donor B - PBS Control - mAdbID:107165
Relations
BioProject PRJNA256332

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Supplementary file Size Download File type/resource
GSE59837_RAW.tar 860.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table
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