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Series GSE5998 Query DataSets for GSE5998
Status Public on Oct 31, 2006
Title Identification of SRF binding sites in Three Human Cell types
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by genome tiling array
Summary We used chromatin immunoprecipitation (ChIP) in combination with human promoter microarrays to identify 216 putative SRF binding sites in the human genome. We performed independent quantitative PCR validation at over half of these sites that resulted in 146 sites we assert to be true binding sites at over 90% confidence. Nearly half of the sites are bound by SRF in only one of the three cell types we tested, providing strong evidence for the diverse roles for SRF in different cell types.
Keywords: ChIP-chip
 
Overall design SRF binding was tested in three different cell lines. It was validated by qPCR. Using the proximal promoter arrays, we are assaying binding in approximately 19,000 human promoters.
 
Citation(s) 17200232
Submission date Oct 09, 2006
Last update date Mar 16, 2012
Contact name Sara Cooper
Organization name Stanford University
Department Genetics
Street address 300 Pasteur Drive
City Stanford
State/province CA
ZIP/Postal code 94305
Country USA
 
Platforms (2)
GPL4089 Agilent-013902 Human Proximal Promoter ChIP-on-Chip Set, Microarray 1 of 2 G4481A
GPL4090 Agilent-013903 Human Proximal Promoter ChIP-on-Chip Set, Microarray 2 of 2 G4481A
Samples (20)
GSM139286 Serum Response Factor ChIP tiling array
GSM139288 SRF 24 hour serum induction, replicate 1, array b Jurkat
GSM139289 SRF 24 hour serum induction, replicate 2, array a Jurkat
Relations
BioProject PRJNA97491

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE5998_RAW.tar 209.3 Mb (http)(custom) TAR (of TXT)

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