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Status |
Public on Aug 01, 2016 |
Title |
Impact of E2 ubiquitin conjugating activity of MUC1 on gene expression |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by array
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Summary |
We identified a novel E2 ubiquitin conjugating activity within the C-terminus domain of MUC1. To determine if E2 ubiquitin conjugating activity of MUC1 is responsible for regulating expression of oncogenic genes we isolated RNA from vector control cells, MUC1 wildtype overexpression cells, and cells that express a serine mutant lacking E2 activity.
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Overall design |
RNA was isolated from each of the 3 cell lines, S2013.Neo, S2013.MUC1-WT, and S2013.MUC1-Ser. Gene expression was analyzed by spotted microarray and the differences in gene expression were calculated between the cell lines
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Contributor(s) |
Brown RB, Hanson RL, Grunkemeyer JA, Fink DM, Radhakrishnan P, Mohr AM, Grandgenett PM, Singh PK, Hollingsworth MA |
Citation missing |
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Submission date |
Aug 12, 2014 |
Last update date |
Aug 02, 2016 |
Contact name |
Ryan Hanson |
E-mail(s) |
ryan.hanson@unmc.edu
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Organization name |
University of Nebraska Medical Center
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Department |
Eppley
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Lab |
Dr. Michael A Hollingsworth
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Street address |
42nd and Emile
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City |
Omaha |
State/province |
Nebraska |
ZIP/Postal code |
68198 |
Country |
USA |
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Platforms (1) |
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Samples (3) |
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Relations |
BioProject |
PRJNA258057 |