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Status |
Public on Feb 18, 2016 |
Title |
Mnemiopsis leidyi high resolution developmental transcriptomic time-course |
Organism |
Mnemiopsis leidyi |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
Classical embryological studies revealed that during mid-embryogenesis vertebrates show similar morphologies. This “phylotypic stage” has recently received support from transcriptome analyses, which have also detected similar stages in nematodes and arthropods. A conserved stage in these three phyla has led us to ask if all animals pass through a universal definitive stage as a consequence of ancestral constraints on animal development. Previous work has suggested that HOX genes may comprise such a ‘zootypic’ stage, however this hypothetical stage has hitherto resisted systematic analysis. We have examined the embryonic development of ten different animals each of a fundamentally different phylum, including a segmented worm, a flatworm, a roundworm, a water bear, a fruitfly, a sea urchin, a zebrafish, a sea anemone, a sponge, and a comb jelly. For each species, we collected the embryonic transcriptomes at ~100 different developmental stages and analyzed their gene expression profiles. We found dynamic gene expression across all of the species that is structured in a stage like manner. Strikingly, we found that animal embryology contains two dominant modules of zygotic expression in terms of their protein domain composition: one involving proliferation, and a second involving differentiation. The switch between these two modules involves induction of the zootype; which in addition to homeobox containing genes, also involves Wnt and Notch signaling as well as forkhead domain transcription factors. Our results provide a systematic characterization of animal universality and identify the points of embryological constraints and flexibility.
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Overall design |
40 single embryo samples
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Contributor(s) |
Levin M, Anavy L, Yanai I |
Citation(s) |
26886793 |
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Submission date |
Aug 18, 2014 |
Last update date |
May 15, 2019 |
Contact name |
Itai Yanai |
E-mail(s) |
yanai@technion.ac.il
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Organization name |
Technion - Israel Institute of Technology
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Department |
Biology
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Lab |
Yanai
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Street address |
Technion City
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City |
Haifa |
ZIP/Postal code |
30200 |
Country |
Israel |
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Platforms (1) |
GPL19089 |
Illumina HiSeq 2000 (Mnemiopsis leidyi) |
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Samples (40)
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This SubSeries is part of SuperSeries: |
GSE70185 |
The mid-developmental transition and the evolution of animal body plans |
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Relations |
BioProject |
PRJNA258375 |
SRA |
SRP045566 |
Supplementary file |
Size |
Download |
File type/resource |
GSE60478_ML_GEO_sample_sheet.tab.gz |
591 b |
(ftp)(http) |
TAB |
GSE60478_ML_exp.tab.gz |
560.7 Kb |
(ftp)(http) |
TAB |
GSE60478_barcodes_umis.tab.gz |
274 b |
(ftp)(http) |
TAB |
SRA Run Selector |
Raw data are available in SRA |
Processed data are available on Series record |
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