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| Status |
Public on Feb 02, 2015 |
| Title |
RNA-seq characterization of an infection of Mycobacterium smegmatis by a cluster A4 mycobacteriophage |
| Organisms |
Mycolicibacterium smegmatis; Mycobacterium phage Kampy |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We present transcriptomic analysis of an infection by a cluster A4 mycobacteriophage of its host, Mycobacterium smegmatis, at six time points (n = 2). Viral transcription is detectable 5 minutes into infection, and viral mRNA makes up the vast majority of all transcription by 30 minutes. Kampy, like many phages, has a genome organized into two arms bearing early- and late-phase genes. We identify two transcriptional start sites (TSS’s) consistent with an early promoter driving expression of half of the genome, along with a second, late-stage promoter that becomes active around 30 minutes after the start of infection.
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| Overall design |
One timepoint before and five timepoints during infection were analyzed using an Ion Torrent PGM. Two biological replicates were sequenced for each timepoint.
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| Contributor(s) |
Halleran A, Clamons S, Saha M |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Aug 21, 2014 |
| Last update date |
May 15, 2019 |
| Contact name |
Margaret Saha |
| E-mail(s) |
mssaha@wm.edu
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| Phone |
757-221-2407
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| Organization name |
College of William and Mary
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| Street address |
540 Landrum Drive
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| City |
Williamsburg |
| State/province |
VA |
| ZIP/Postal code |
23185 |
| Country |
USA |
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| Platforms (2) |
| GPL19104 |
Ion Torrent PGM (Mycobacterium smegmatis) |
| GPL19106 |
Ion Torrent PGM (Mycobacterium phage Kampy; Mycobacterium smegmatis) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA258602 |
| SRA |
SRP045668 |
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