NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Series GSE60696 Query DataSets for GSE60696
Status Public on Dec 31, 2023
Title Transcriptome analysis of zebrafish exposed to neomycin and/or tacrine
Organism Danio rerio
Experiment type Expression profiling by array
Summary Aminoglycosides remain in current use because of the benefits, including the broad-spectrum, inexpensiveness to produce, and a relatively small incidence of allergic reactions. However, aminoglycosides may induce irreversible hearing loss. Recently, tacrine, an acetylcholineesterase inhibitor originally developed to treat Alzheimer’s disease, was found to be protective to neomycin-induced hair cell damage in zebrafish and mouse. However, the protection mechanism remain to be largely unknown. In this study, we revealed that tacrine significantly suppressed the expression of tnf mRNA and ROS production induced by neomycin. Reduction of tnf expression by the antisense morpholino or transcription activator-like effectors nuclease significantly suppressed the hair cell damage but not ROS production induced by neomycin. In zebrafish treated with an antagonist of NMDA receptor MK-801 or a mitochondrial ROS inhibitor acetyl-L-carnitine, ROS production, tnf expression, and hair cell damage induced by neomycin were also significantly reduced. These levels of suppression were not significantly different from those of co-treatment of tacrine and MK-801 or acetyl-L-carnitine. These findings suggest that activation of NMDA receptor followed by ROS production and subsequent tnf expression are involved in neomycin-induced hair cell damage and that tacrine ameliorate neomycin-induced hair cell damage through inhibition of the signaling pathway.
 
Overall design Gene expression regulated by neomycin and/or tacrine in zebrafish at 5 dpf was measured. Three independent experiments were performed for each group.
 
Contributor(s) Nishimura Y, Kondo T, Tanaka T
Citation missing Has this study been published? Please login to update or notify GEO.
Submission date Aug 25, 2014
Last update date Dec 31, 2023
Contact name Yuhei Nishimura
E-mail(s) yuhei@med.mie-u.ac.jp
Phone 81-59-231-5411
Organization name Mie University Graduate School of Medicine
Department Integrative Pharmacology
Street address 2-174 Edobashi
City Tsu
State/province Mie
ZIP/Postal code 514-8507
Country Japan
 
Platforms (1)
GPL14664 Agilent-026437 D. rerio (Zebrafish) Oligo Microarray V3 (Probe Name version)
Samples (9)
GSM1486019 control_rep1
GSM1486020 control_rep2
GSM1486021 control_rep3
Relations
BioProject PRJNA259316

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE60696_RAW.tar 16.7 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap
External link. Please review our privacy policy.