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Series GSE60819 Query DataSets for GSE60819
Status Public on Aug 28, 2014
Title Effects of acute prenatal exposure to ethanol on microRNA expression are ameliorated by environmental manipulation.
Platform organisms Rattus norvegicus; synthetic construct
Sample organism Rattus norvegicus
Experiment type Expression profiling by array
Non-coding RNA profiling by array
Summary In this study, we tested if miRNAs are altered in amygdala and ventral striatum as a consequence of prenatal ethanol exposure and/or social enrichment. miRNA samples from 72 male and female adolescent rats were analyzed by RNA-Seq analysis and Affymetrix miRNA arrays. Several miRNAs showed significant changes due to prenatal ethanol exposure or social enrichment in one or both brain regions. Some of the miRNA changes caused by ethanol were reversed by social enrichment. The top predicted gene targets of these miRNAs were mapped and subjected to pathway enrichment analysis. We also directly examined the evidence for modulation of target mRNAs in whole transcriptome microarray data from the same rats. Among the pathways most strongly affected were p53, CREB, Glutamate and GABA signaling. Together, our data suggest a number of novel epigenetic mechanisms for social enrichment to reverse the effects of ethanol exposure.
 
Overall design A total of 48 Rat ST Gene 1.0 GeneChips and 48 miRNA 2.0 GeneChips were run on RNA purified from 2 brain regions (amygdala and ventral striatum) of postnatal day 42 Long Evans rats. 3 replicate arrays were run for each gender within 4 different treatment groups (prenatal ethanol or prenatal saline exposed; postnatal socially-enriched or non-enriched environment)
 
Contributor(s) Middleton FA, Mooney S, Ignacio C
Citation(s) 25309888
Submission date Aug 27, 2014
Last update date Jan 08, 2018
Contact name Frank A Middleton
E-mail(s) middletf@upstate.edu
Phone 315-464-7721
Organization name SUNY Upstate Medical Univ
Department Neuroscience and Physiology
Street address 505 Irving Avenue
City Syracuse
State/province NY
ZIP/Postal code 13210
Country USA
 
Platforms (2)
GPL6247 [RaGene-1_0-st] Affymetrix Rat Gene 1.0 ST Array [transcript (gene) version]
GPL14613 [miRNA-2] Affymetrix Multispecies miRNA-2 Array
Samples (96)
GSM1489316 female saline non-enriched ventral striatum replicate 1
GSM1489317 female saline non-enriched ventral striatum replicate 2
GSM1489318 female saline non-enriched ventral striatum replicate 3
This SubSeries is part of SuperSeries:
GSE60901 Comparison of small RNA-seq and microarray analysis for determining the effects of acute prenatal ethanol exposure on microRNA expression and its amelioration by environmental manipulation
Relations
BioProject PRJNA259613

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE60819_RAW.tar 250.3 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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